Plants are attractive alternative expression hosts for the production of recombinant proteins.
Many therapeutic proteins are glycosylated with N-and O-glycosylation being the most …

Asparagine (N)-linked protein glycosylation is a ubiquitous co-and posttranslational
modification which has a huge impact on the biogenesis and function of proteins and …

Recombinantly produced proteins are indispensable tools for medical applications. Since
the majority of them are glycoproteins, their N-glycosylation profiles are major determinants …

Control over glycosylation is an important quality parameter in recombinant protein
production. Here, we demonstrate the generation of a marker‐free genome edited Nicotiana …

The processing of N-linked oligosaccharides in the secretory pathway requires the
sequential action of a number of glycosidases and glycosyltransferases. We studied the …

O-Linked glycans vary between eukaryotic cell types and play an important role in
determining a glycoprotein's properties, including stability, target recognition, and potentially …

Sialyl galactose (Sia-Gal) is one of the most abundant terminal motifs of oligosaccharides
and is widely present in glycoconjugates and unconjugated glycans of animals. To …

The commercial production of complex pharmaceutical proteins from human origin in plants
is currently limited through differences in protein N-glycosylation pattern between plants and …

Highlights•Plants glycosylate proteins in a largely homogeneous way.•We report extensive
glycan engineering in Nitotiana benthamiana.•Recombinant proteins produced in …

Plant N‐linked glycans differ substantially from their mammalian counterparts, mainly with
respect to modifications of the core glycan, which typically contains a β (1, 2)‐xylose and an …