Asparagine (N)-linked protein glycosylation is a ubiquitous co-and post-translational
modification which can alter the biological function of proteins and consequently affects the …

Cell-free, chemoenzymatic platforms are emerging technologies towards generating
glycoconjugates with defined and homogeneous glycoforms. Recombinant …

Endo-M, an endo-β-N-acetylglucosaminidase from Mucor hiemalis, is a family 85 glycoside
hydrolase. This enzyme is unique in that it can transfer en bloc the oligosaccharide of …

Gaucher disease is an inherited lysosomal storage disorder caused by a deficiency of
functional enzyme β-glucocerebrosidase (GCase). Recombinant GCase has been used in …

Mucin-type O-glycosylation is an important post-translational modification that confers a
variety of biological properties and functions to proteins. This post-translational modification …

Glycoengineering of plant expression systems is a prerequisite for the production of
biopharmaceuticals that are compatible with animal-derived glycoproteins. Large amounts …

Hyperglycosylated proteins are more stable, show increased serum half-life and less
sensitivity to proteolysis compared to non-sialylated forms. This applies particularly to …

Secretions of parasitic worms (helminths) contain a wide collection of immunomodulatory
glycoproteins with the potential to treat inflammatory disorders, like autoimmune diseases …

In our previous study, we found unique free N-glycans (FNGs), which carry a single GlcNAc
residue (GN1) at the reducing-end side and the Lewis-a epitope at the non-reducing-end …

N-glycans on the cell surface provide distinct signatures that are recognized by different
glycan-binding proteins (GBPs) and pathogens. Most glycans in humans are asymmetric …