Recent methodological advancements in microscopy and DNA sequencing-based methods
provide unprecedented new insights into the spatio-temporal relationships between …

We studied the nuclear topography of RNA transcription and DNA replication in mammalian
cell types with super-resolution fluorescence microscopy, which offers a resolution beyond …

Advances in the specific fluorescent labeling of chromatin in fixed and living human cells in
combination with three-dimensional (3D) and 4D (space plus time) fluorescence microscopy …

The nuclear architecture is considered an important contributor to genome function.
Although the fine structural features of the cell nucleus have been investigated extensively …

Ultrastructural cytochemistry has been, for many years now, a major tool for investigating
structure–function relationships in the cell nucleus. It has been essential in approaching the …

Abstract The 4D Nucleome Network aims to develop and apply approaches to map the
structure and dynamics of the human and mouse genomes in space and time with the goal …

Ribosomal RNA (rRNA) transcription by RNA polymerase I (Pol I) is the first key step of
ribosome biogenesis. While the molecular mechanisms of rRNA transcription regulation …

This work reviews nuclear compartments, defined broadly to include distinct nuclear
structures, bodies, and chromosome domains. It first summarizes original cytological …

The organization of the genome in the nucleus of a eukaryotic cell is fairly complex and
dynamic. Various features of the nuclear architecture, including compartmentalization of …

Abstract 4D nucleome research aims to understand the impact of nuclear organization in
space and time on nuclear functions, such as gene expression patterns, chromatin …