Microinjection of foreign DNA into pronuclei of a fertilized oocyte has predominantly been
used for the generation of transgenic livestock. This technology works reliably, but is …

The first transgenic livestock species were reported in 1985. Since then microinjection of
foreign DNA into pronuclei of zygotes has been the method of choice. It is now being …

The ability to transfer (trans) genes into the germline of domestic animals is both exciting
and challenging. Many believe that this biotechnology has huge potential to benefit both …

Nuclear transfer (NT) is a procedure by which genetically identical individuals can be
created. The technology of pig somatic NT, including in vitro maturation of oocytes, isolation …

Pigs are increasingly being recognized as good large-animal models for translational
research, linking basic science to clinical applications in order to establish novel …

NUCLEAR TRANSFER is a procedure by which genetically identical individuals can be
created. The applications of these techniques for nuclear transfer will be in agricultural …

Microinjection of DNA constructs into pronuclei of zygotes has been the method of choice for
the generation of transgenic livestock. However, this procedure is characterized by low …

Progress in genetic engineering over the past few decades has made it possible to develop
methods that have led to the production of transgenic animals. The development of …

During the past 6 years, considerable advancement has been achieved in experimental
embryology of pigs. This process was mainly generated by the rapidly increasing need for …

Initial technologies for creating transgenic swine only permitted random integration of the
construct. However, by combining the technology for homologous recombination in fetal …