[HTML][HTML] ATP Binding to the σ54-Dependent Activator XylRTriggers a Protein Multimerization Cycle Catalyzed by UAS DNA

J Pérez-Martín, V de Lorenzo - Cell, 1996 - cell.com
The events that take place at the prokaryotic enhancer of the Pu promoter of Pseudomonas
putida prior to the engagement of the σ 54-RNA polymerase (σ 54-RNAP) have been …

[HTML][HTML] Recruitment of RNA Polymerase Is a Rate-limiting Step for the Activation of the ς54 Promoter Pu ofPseudomonas putida

M Carmona, V de Lorenzo, G Bertoni - Journal of Biological Chemistry, 1999 - ASBMB
The activity of the ς 54-promoter Pu of Pseudomonas putida was examined in vitro with a
DNA template lacking upstream activating sequences, such that RNA polymerase can be …

[HTML][HTML] Active recruitment of σ54-RNA polymerase to the Pu promoter of Pseudomonas putida: role of IHF and αCTD

G Bertoni, N Fujita, A Ishihama, V de Lorenzo - The EMBO journal, 1998 - embopress.org
The sequence elements determining the binding of the σ 54-containing RNA polymerase (σ
54-RNAP) to the Pu promoter of Pseudomonas putida have been examined. Contrary to …

In vitro activities of an N-terminal truncated form of XylR, a σ54-dependent transcriptional activator of Pseudomonas putida

J Pérez-Martı́n, V de Lorenzo - Journal of molecular biology, 1996 - Elsevier
A truncated derivative of the XylR protein, which is able to constitutively activate the σ54-
dependentPupromoter of the TOL (toluene biodegradation) plasmid ofPseudomonas putida …

Roles of effectors in XylS-dependent transcription activation: intramolecular domain derepression and DNA binding

P Domínguez-Cuevas, P Marín, S Busby… - Journal of …, 2008 - Am Soc Microbiol
XylS, an AraC family protein, activates transcription from the benzoate degradation pathway
Pm promoter in the presence of a substrate effector such as 3-methylbenzoate (3MB). We …

Changes in conserved region 2 of Escherichia coli σ70 affecting promoter recognition

C Waldburger, T Gardella, R Wong… - Journal of molecular …, 1990 - Elsevier
We describe a mutation in rpoD, the gene encoding the σ 70 subunit of RNA polymerase,
which alters the promoter specificity of the holoenzyme in vivo. The mutant σ causes a …

Integration host factor suppresses promiscuous activation of the sigma 54-dependent promoter Pu of Pseudomonas putida.

J Pérez-Martín, V De Lorenzo - Proceedings of the National …, 1995 - National Acad Sciences
In the presence of m-xylene, the Pu promoter of the TOL plasmid of Pseudomonas putida is
activated by the prokaryotic enhancer-binding protein XylR. The intervening DNA segment …

[HTML][HTML] Recruitment of σ54-RNA polymerase to the Pu promoter of Pseudomonas putida through integration host factor-mediated positioning switch of α subunit …

R Macchi, L Montesissa, K Murakami… - Journal of Biological …, 2003 - ASBMB
The interactions between the σ 54-containing RNA polymerase (σ 54-RNAP) and the region
of the Pseudomonas putida Pu promoter spanning from the enhancer to the binding site for …

Evidence for a tyrosine–adenine stacking interaction and for a short-lived open intermediate subsequent to initial binding of Escherichia coli RNA polymerase to …

LA Schroeder, TJ Gries, RM Saecker… - Journal of molecular …, 2009 - Elsevier
Bacterial RNA polymerase and a “sigma” transcription factor form an initiation-competent
“open” complex at a promoter by disruption of about 14 base pairs. Strand separation is …

Activation and repression of a σN‐dependent promoter naturally lacking upstream activation sequences

O Porrúa, V García‐González, E Santero… - Molecular …, 2009 - Wiley Online Library
The Pseudomonas sp. strain ADP protein AtzR is a LysR‐type transcriptional regulator
required for activation of the atzDEF operon in response to nitrogen limitation and cyanuric …