Tethering σ70 to RNA polymerase reveals high in vivo activity of σ factors and σ70-dependent pausing at promoter-distal locations

RA Mooney, R Landick - Genes & development, 2003 - genesdev.cshlp.org
Bacterial σ factors compete for binding to RNA polymerase (RNAP) to control promoter
selection, and in some cases interact with RNAP to regulate at least the early stages of …

Coupling nucleotide hydrolysis to transcription activation performance in a bacterial enhancer binding protein

N Joly, M Rappas, SR Wigneshweraraj… - Molecular …, 2007 - Wiley Online Library
The bacterial enhancer binding proteins (bEBP) are members of the AAA+ protein family
and have a highly conserved 'DE'Walker B motif thought to be involved in the catalytic …

Interaction of the sigma factor and the nusA gene protein of E. coli with RNA polymerase in the initiation-termination cycle of transcription

J Greenblatt, J Li - Cell, 1981 - cell.com
The nu. sA gene protein of E. coli is involved in regulating termination of transcription in vivo.
In vitro it causes termination of transcription in the tR2 region of the PR operon of …

Role of σs in transcription from the positively controlled Pm promoter of the TOL plasmid of Pseudomonas putida

S Marqués, MT Gallegos, JL Ramos - Molecular microbiology, 1995 - Wiley Online Library
Transcription from the TOL plasmid Pm promoter is dependent on the XylS regulator
activated by benzoate effectors. We analysed transcription from Pm in several backgrounds …

Communication between Eσ54, promoter DNA and the conserved threonine residue in the GAFTGA motif of the PspF σ54‐dependent activator during transcription …

P Bordes, SR Wigneshweraraj, M Chaney… - Molecular …, 2004 - Wiley Online Library
Conversion of Eσ54 closed promoter complexes to open promoter complexes requires
specialized activators which are members of the AAA (ATPases Associated with various …

ATP ground-and transition states of bacterial enhancer binding AAA+ ATPases support complex formation with their target protein, σ54

B Chen, M Doucleff, DE Wemmer, S De Carlo… - Structure, 2007 - cell.com
Transcription initiation by the σ54 form of bacterial RNA polymerase requires hydrolysis of
ATP by an enhancer binding protein (EBP). We present SAS-based solution structures of the …

Mechanism of bacterial transcription initiation: RNA polymerase-promoter binding, isomerization to initiation-competent open complexes, and initiation of RNA …

RM Saecker, MT Record Jr, PL Dehaseth - Journal of molecular biology, 2011 - Elsevier
Initiation of RNA synthesis from DNA templates by RNA polymerase (RNAP) is a multi-step
process, in which initial recognition of promoter DNA by RNAP triggers a series of …

The sigma 54-dependent promoter Ps of the TOL plasmid of Pseudomonas putida requires HU for transcriptional activation in vivo by XylR

J Pérez-Martín, V de Lorenzo - Journal of bacteriology, 1995 - Am Soc Microbiol
In the presence of toluene and xylenes, the sigma 54-dependent Ps promoter of the TOL
(toluene biodegradation) plasmid pWW0 of Pseudomonas putida is activated at a distance …

Positive activation of gene expression

VA Rhodius, SJW Busby - Current opinion in microbiology, 1998 - Elsevier
Most bacterial transcription activators function by making direct contact with RNA
polymerase at target promoters. Some activators contact the carboxy-terminal domain of the …

Bacterial enhancer-binding proteins: unlocking σ54-dependent gene transcription

M Rappas, D Bose, X Zhang - Current opinion in structural biology, 2007 - Elsevier
Bacterial transcription relies on the binding of dissociable sigma (σ) factors to RNA
polymerase (RNAP) for promoter specificity. The major variant sigma factor (σ54) forms a …