A postcolumn immunochemical detection system for on-line coupling to HPLC is described.
The effluent from a reversed-phase LC column is mixed with fluorescein-labelled antibodies …

All the bioanalytical labeling and detection techniques in one source! This book gathers
together all the important nonradioactive labeling techniques for nucleic acids, proteins …

Abstract N 4-[N-(6-trifluoroacetylamidocaproyl)-2-aminoethyl]-5′-O-dimethoxytrityl-5-methyl-
2′-deoxycytidine-3′-N, N-diisopropyl-methylphosphoramidite has been synthesized. This …

A simple approach to DNA tail-labelling using terminal deoxynucleotidyl transferase and
modified deoxynucleoside triphosphates is presented. Amino-and nitrophenyl-modified …

The syntheses are described of two types of linker molecule useful for the specific
attachment of non-radioactive labels such as biotin and fluorophores to the 5′ terminus of …

Terminal deoxynucleotidyl transferase was used to add labeled dAMP residues to the 3′
end of oligonucleotide probes that hybridize to the 5′ end of the neomycin …

Digoxigenin-labeled nucleic acid probes for angiotensin-converting enzyme were prepared
by the polymerase chain reaction. In a model system, these probes were used to optimize …

In situ hybridization (ISH) techniques have proven to be invaluable tools for diagnostic
molecular laboratories in the detection of gene expression and viral infection in cell and …

We describe a simple, efficient, procedure for labeling oligonucleotides to high specific
activity (> 1× 10 8 cpm/μmol) by hydrogen exchange with tritlated water at the C8 positions …

This paper deals with the chemical synthesis of natural and modified
oligodeoxyribonucleotides and chimeric oligoribo-oligodeoxyribo-nucleotides. The first part …