Application of CRISPR-Cas9 systems has revolutionized genome editing across all domains
of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in …

CRISPR-Cas9 has been demonstrated as a transformative genome engineering tool for
many eukaryotic organisms; however, its utilization in bacteria remains limited and …

Clostridium butyricum has been widely used as a probiotic for humans and food animals.
However, the mechanisms of beneficial effects of C. butyricum on the host remain poorly …

The CRISPR-Cas9 system is a powerful and revolutionary genome-editing tool for
eukaryotic genomes, but its use in bacterial genomes is very limited. Here, we investigated …

CRISPR/Cas-based genetic engineering has revolutionised molecular biology in both
eukaryotes and prokaryotes. Several tools dedicated to the genomic transformation of the …

CRISPR-Cas systems are now widely used for genome editing and transcriptional
regulation in diverse organisms. The compact and portable nature of class 2 single effector …

Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …

CRISPR-Cas systems adapt “memories” via spacers from viruses and plasmids to develop
adaptive immunity against mobile genetic elements. Mature CRISPR RNAs guide CRISPR …

Cpf1 represents a novel single RNA‐guided CRISPR/Cas endonuclease system suitable for
genome editing with distinct features compared with Cas9. We demonstrate the functionality …

The targeting of nucleases to specific DNA sequences facilitates genome editing. Recent
work demonstrated that the CRISPR-associated (Cas) nuclease Cas9 can be targeted to …