High throughput and quantitative measurement of microbial metabolome by gas chromatography/mass spectrometry using automated alkyl chloroformate …

L Zhao, Y Ni, M Su, H Li, F Dong, W Chen… - Analytical …, 2017 - ACS Publications
The ability to identify and quantify small molecule metabolites derived from gut microbial–
mammalian cometabolism is essential for the understanding of the distinct metabolic …

Microbial metabolomics with gas chromatography/mass spectrometry

MM Koek, B Muilwijk, MJ van der Werf… - Analytical …, 2006 - ACS Publications
An analytical method was set up suitable for the analysis of microbial metabolomes,
consisting of an oximation and silylation derivatization reaction and subsequent analysis by …

Analytical platform for metabolome analysis of microbial cells using methyl chloroformate derivatization followed by gas chromatography–mass spectrometry

KF Smart, RBM Aggio, JR Van Houtte… - Nature protocols, 2010 - nature.com
This protocol describes an analytical platform for the analysis of intra-and extracellular
metabolites of microbial cells (yeast, filamentous fungi and bacteria) using gas …

Chemoselective and highly sensitive quantification of gut microbiome and human metabolites

W Lin, LP Conway, M Vujasinovic, JM Löhr… - Angewandte …, 2021 - Wiley Online Library
The microbiome has a fundamental impact on the human host's physiology through the
production of highly reactive compounds that can lead to disease development. One class of …

Mass spectrometry-based metabolomics in microbiome investigations

A Bauermeister, H Mannochio-Russo… - Nature Reviews …, 2022 - nature.com
Microbiotas are a malleable part of ecosystems, including the human ecosystem.
Microorganisms affect not only the chemistry of their specific niche, such as the human gut …

Coupling targeted and untargeted mass spectrometry for metabolome-microbiome-wide association studies of human fecal samples

AV Melnik, RR da Silva, ER Hyde, AA Aksenov… - Analytical …, 2017 - ACS Publications
Increasing appreciation of the gut microbiome's role in health motivates understanding the
molecular composition of human feces. To analyze such complex samples, we developed a …

[HTML][HTML] New enzymatic and mass spectrometric methodology for the selective investigation of gut microbiota-derived metabolites

C Ballet, MSP Correia, LP Conway, TL Locher… - Chemical …, 2018 - pubs.rsc.org
Gut microbiota significantly impact human physiology through metabolic interaction.
Selective investigation of the co-metabolism of bacteria and their human host is a …

[HTML][HTML] Unlocking the secrets of the microbiome: exploring the dynamic microbial interplay with humans through metabolomics and their manipulation for synthetic …

H Muhamadali, CL Winder, WB Dunn… - Biochemical …, 2023 - portlandpress.com
Metabolomics is a powerful research discovery tool with the potential to measure hundreds
to low thousands of metabolites. In this review, we discuss the application of GC–MS and LC …

Expanding coverage of the metabolome for global metabolite profiling

O Yanes, R Tautenhahn, GJ Patti… - Analytical chemistry, 2011 - ACS Publications
Mass spectrometry-based metabolomics is the comprehensive study of naturally occurring
small molecules collectively known as the metabolome. Given the vast structural diversity …

Optimization of a sample preparation method for the metabolomic analysis of clinically relevant bacteria

R Marcinowska, J Trygg, H Wolf-Watz, T Mortiz… - Journal of …, 2011 - Elsevier
Metabolomics, or metabolite profiling, is an approach that is increasingly used to study the
metabolism of diverse organisms, elucidate biological processes and/or find characteristic …