Yeast display platform with expression of linear peptide epitopes for high-throughput assessment of peptide-MHC-II binding
BD Huisman, PA Balivada, ME Birnbaum - Journal of Biological Chemistry, 2023 - ASBMB
Yeast display can serve as a powerful tool to assess the binding of peptides to the major
histocompatibility complex (pMHC) and pMHC-T-cell receptor binding. However, this …
histocompatibility complex (pMHC) and pMHC-T-cell receptor binding. However, this …
Yeast display platform for expression of linear peptide epitopes to assess peptide-MHC-II binding in high-throughput
BD Huisman, PA Balivada, ME Birnbaum - bioRxiv, 2022 - biorxiv.org
Yeast display can serve as a powerful tool to assess peptide-MHC (pMHC) and pMHC-TCR
binding. However, this approach is often limited by the need to optimize MHC proteins for …
binding. However, this approach is often limited by the need to optimize MHC proteins for …
Yeast display for the identification of peptide-MHC ligands of immune receptors
T cells detect peptide antigens presented by major histocompatibility complex (MHC)
proteins via their T cell receptor (TCR). The sequence diversity of possible antigens, with …
proteins via their T cell receptor (TCR). The sequence diversity of possible antigens, with …
RIPPA: Identification of MHC‐II Binding Peptides from Antigen Using a Yeast Display‐Based Approach
Mapping MHC‐II binding peptides derived from an antigenic protein for potential CD4+ T‐
cell epitopes has been challenging due to a lack of experimental approaches that are both …
cell epitopes has been challenging due to a lack of experimental approaches that are both …
High-throughput engineering and analysis of peptide binding to class II MHC
Class II major histocompatibility complex (MHC-II) proteins govern stimulation of adaptive
immunity by presenting antigenic peptides to CD4+ T lymphocytes. Many allelic variants of …
immunity by presenting antigenic peptides to CD4+ T lymphocytes. Many allelic variants of …
Yeast surface display of a noncovalent MHC class II heterodimer complexed with antigenic peptide
ET Boder, JR Bill, AW Nields… - Biotechnology and …, 2005 - Wiley Online Library
Microbial protein display technologies have enabled directed molecular evolution of binding
and stability properties in numerous protein systems. In particular, dramatic improvements to …
and stability properties in numerous protein systems. In particular, dramatic improvements to …
T cell receptor engineering and analysis using the yeast display platform
The αβ heterodimeric T cell receptor (TCR) recognizes peptide antigens that are transported
to the cell surface as a complex with a protein encoded by the major histocompatibility …
to the cell surface as a complex with a protein encoded by the major histocompatibility …
Cell surface display of functional human MHC class II proteins: yeast display versus insect cell display
Reliable and robust systems for engineering functional major histocompatibility complex
class II (MHCII) proteins have proved elusive. Availability of such systems would enable the …
class II (MHCII) proteins have proved elusive. Availability of such systems would enable the …
Repertoire-scale determination of class II MHC peptide binding via yeast display improves antigen prediction
CD4+ helper T cells contribute important functions to the immune response during pathogen
infection and tumor formation by recognizing antigenic peptides presented by class II major …
infection and tumor formation by recognizing antigenic peptides presented by class II major …
A yeast display system for engineering functional peptide-MHC complexes
SE Brophy, PD Holler, DM Kranz - Journal of immunological methods, 2003 - Elsevier
In a cellular immune response, antigenic peptides derived by intracellular processing of
foreign pathogens are bound to the class I major histocompatability complex (MHC I) and …
foreign pathogens are bound to the class I major histocompatability complex (MHC I) and …