Tn5 is a composite transposon consisting of two IS50 sequences in inverted orientation with
respect to a unique, central region encoding several antibiotic resistances. The IS50R …

The transposase (Tnp) of the bacterial transposon Tn5 acts 50-to 100-fold more efficiently on
elements located cis to the site of its synthesis compared with those located in trans. In an …

The element IS50 encodes all Tn5 functions required for transposition and all regulatory
mechanisms. Bacterial transposon Tn5 encodes two proteins, transposase and the …

Tn 5 is a composite bacterial transposon that encodes a protein, transposase (Tnp), required
for movement of the transposon. The initial step in the transposition pathway involves …

Transposition of Tn5 and of its component insertion sequence IS50R is regulated through
the action of two proteins it encodes: a cis-acting transposase, Tnp, and a trans-acting …

We have purified TnsB, a transposition protein encoded by the bacterial transposon Tn7.
The purification procedure involves three chromatographic steps (DNA-cellulose, norleucine …

The transposase encoded by the tnpA gene of Tn3 is a protein specifically required for Tn3
transposition. We have purified it to homogeneity from an Escherichia coli strain containing a …

The binding of transposase (Tnp) to the specific Tn5 end sequences is the first dedicated
reaction during transposition. In this study, comparative DNA-binding analyses were …

The end sequences of the IS50 insertion sequence are known as the outside end (OE) and
inside end. These complex ends are related but nonidentical 19-bp sequences that serve as …

Tn5 is an excellent model system for understanding the molecular basis of DNA‐mediated
transposition. Mechanistic information has come from genetic and biochemical …