Electrochemical modification of polypeptides at selenocysteine
AS Mackay, JWC Maxwell, MJ Bedding… - Angewandte …, 2023 - Wiley Online Library
Mild strategies for the selective modification of peptides and proteins are in demand for
applications in therapeutic peptide and protein discovery, and in the study of fundamental …
applications in therapeutic peptide and protein discovery, and in the study of fundamental …
Rapid additive-free selenocystine–selenoester peptide ligation
We describe an unprecedented reaction between peptide selenoesters and peptide dimers
bearing N-terminal selenocystine that proceeds in aqueous buffer to afford native amide …
bearing N-terminal selenocystine that proceeds in aqueous buffer to afford native amide …
Diselenide–selenoester ligation for chemical protein synthesis
Chemoselective peptide ligation methods have provided synthetic access to numerous
proteins, including those bearing native post-translational modifications and unnatural …
proteins, including those bearing native post-translational modifications and unnatural …
Expressed protein selenoester ligation
Herein, we describe the development and application of a novel expressed protein
selenoester ligation (EPSL) methodology for the one‐pot semi‐synthesis of modified …
selenoester ligation (EPSL) methodology for the one‐pot semi‐synthesis of modified …
Synthesis and semisynthesis of selenopeptides and selenoproteins
Highlights•When selenoester is used for ligation reactions with diselenides, kinetics are
accelerated and side reactions are minimized.•Selenozolidine is emerging as the preferred …
accelerated and side reactions are minimized.•Selenozolidine is emerging as the preferred …
Utilizing selenocysteine for expressed protein ligation and bioconjugations
J Liu, Q Chen, S Rozovsky - Journal of the American Chemical …, 2017 - ACS Publications
Employing selenocysteine-containing protein fragments to form the amide bond between
respective protein fragments significantly extends the current capabilities of the widely used …
respective protein fragments significantly extends the current capabilities of the widely used …
Solid-phase synthesis of peptide selenoesters via a side-chain anchoring strategy
CC Hanna, SS Kulkarni, EE Watson… - Chemical …, 2017 - pubs.rsc.org
Peptide selenoesters have recently emerged as key building blocks for the ligation-based
assembly of large polypeptides and proteins. Herein, we report an efficient solid-phase …
assembly of large polypeptides and proteins. Herein, we report an efficient solid-phase …
Preparation of Peptide Selenoesters from Their Corresponding Acyl Hydrazides†
Y Li, J Liu, Q Zhou, J Zhao… - Chinese Journal of …, 2021 - Wiley Online Library
Main observation and conclusion Selenoesters are useful substitutes for traditional
thioesters in protein ligation chemistry due to their high reactivity in the trans‐thio …
thioesters in protein ligation chemistry due to their high reactivity in the trans‐thio …
Peptide ligation chemistry at selenol amino acids
The convergent assembly of peptide fragments by native chemical ligation has
revolutionized the way in which proteins can be accessed by chemical synthesis. A variation …
revolutionized the way in which proteins can be accessed by chemical synthesis. A variation …
Site‐Selective Modification of Peptides and Proteins via Interception of Free‐Radical‐Mediated Dechalcogenation
RC Griffiths, FR Smith, JE Long… - Angewandte Chemie …, 2020 - Wiley Online Library
The development of site‐selective chemistry targeting the canonical amino acids enables
the controlled installation of desired functionalities into native peptides and proteins. Such …
the controlled installation of desired functionalities into native peptides and proteins. Such …