[HTML][HTML] Generation of rodent and human osteoblasts
This paper describes the isolation, culture and staining of primary osteoblasts from neonatal
rodents and human samples. The calvaria and long-bone assays allow direct measurement …
rodents and human samples. The calvaria and long-bone assays allow direct measurement …
Primary human osteoblast cultures
JP Dillon, VJ Waring-Green, AM Taylor… - Bone research …, 2012 - Springer
Osteoblast cultures can be used to investigate the mechanisms of bone formation, to probe
the cellular and molecular basis of bone disease, and to screen for potential therapeutic …
the cellular and molecular basis of bone disease, and to screen for potential therapeutic …
Isolation and culture of human osteoblasts
A Gartland, RMH Rumney, JP Dillon… - Human cell culture …, 2012 - Springer
The skeleton is a dynamic organ that is constantly active throughout life. The highly
coordinated actions of bone cells early in life determine the body's shape and form, whilst …
coordinated actions of bone cells early in life determine the body's shape and form, whilst …
Human osteoblast culture
JA Gallagher - Bone research protocols, 2003 - Springer
Osteoblasts are the cells responsible for the formation of bone; they synthesize almost all of
the constituents of the bone matrix and direct its subsequent mineralization. Once a phase of …
the constituents of the bone matrix and direct its subsequent mineralization. Once a phase of …
Isolation and culture of neonatal mouse calvarial osteoblasts
JH Jonason, RJ O'Keefe - Skeletal Development and Repair: Methods …, 2014 - Springer
This chapter describes the isolation and culture of neonatal mouse calvarial osteoblasts.
This primary cell population is obtained by sequential enzymatic digestion of the calvarial …
This primary cell population is obtained by sequential enzymatic digestion of the calvarial …
Osteoblast isolation from murine calvaria and long bones
AD Bakker, J Klein-Nulend - Bone research protocols, 2012 - Springer
This chapter describes the isolation of primary mouse osteoblasts from adult mouse calvaria
and long bones, as well as the process of isolation of bone cells from neonatal mouse …
and long bones, as well as the process of isolation of bone cells from neonatal mouse …
Osteoblast lineage
JT Triffitt, ROC Oreffo - 1998 - Elsevier
Publisher Summary This chapter discusses the osteoblast lineage. The advent of cell culture
and molecular biology has seen an explosion in information on the osteoblast lineage, bone …
and molecular biology has seen an explosion in information on the osteoblast lineage, bone …
Isolation and culture of human bone-derived cells
R Gundle, K Stewart, J Screen… - Marrow stromal cell …, 1998 - cambridge.org
Cells of the osteoblast lineage are essential for the normal growth, development and
maintenance of the vertebrate skeleton. They are responsible for bone formation, participate …
maintenance of the vertebrate skeleton. They are responsible for bone formation, participate …
Assessing new bone formation in neonatal calvarial organ cultures
KS Mohammad, JM Chirgwin, TA Guise - Osteoporosis: Methods and …, 2008 - Springer
Bone formation is a complex process, and testing anabolic effects on the skeleton of agents
is slow and expensive in animals. Neonatal mouse calvariae cultured ex vivo show strong …
is slow and expensive in animals. Neonatal mouse calvariae cultured ex vivo show strong …
Osteoblasts
L Di-Silvio, N Gurav - Human cell culture: primary mesenchymal cells, 2001 - Springer
This chapter describes a primary human osteoblast (HOB) cell model, focusing on culture
conditions that favor proliferation and differentiation. Biochemical, light and electron …
conditions that favor proliferation and differentiation. Biochemical, light and electron …