A sequencing method called genomic amplification with transcript sequencing (GAWTS) is
described that is based on amplification with the polymerase chain reaction (PCR). GAWTS …

A rapid and versatile method has been developed for the synthesis of oligonucleotides
which contain an aliphatic amino group at their 5′ terminus. This amino group reacts …

1205 ed sequentially to the 3'end of the primer from the corresponding deoxynucleoside
triphosphates, making a com-plementary copy of the template DNA. By using triphosphates …

A strategy for rapid DNA sequence acquisition in an ordered, nonrandom manner, while
retaining all of the conveniences of the dideoxy method with M13 transducing phage DNA …

Fluorescent dye-labeled DNA primers have been developed that exploit fluorescence
energy transfer (ET) to optimize the absorption and emission properties of the label. These …

A method and instrument for automated DNA sequencing without radioactivity have been
developed. In spite of the success with radioactive labels there are drawbacks attached to …

We report a simple and reliable protocol for nucleotide sequencing using the Sanger
dideoxy technique on linearized double-stranded DNA molecules with specific …

It has been shown previously that deoxyguanosine residues in DNA are hydroxylated at the
C-8 position both in vitro and in vivo to produce 8-hydroxydeoxyguanosine (8-OH-dG) by …

The DNA/RNA Synthesizer provides a complete and automated procedure for the synthesis
of DNA sequences. Each base unit is added in a 30-minute cycle, permitting a tetradecamer …

This report describes the fluorescence hybridization of DNA sequence probes to interphase
nuclei in suspension and the quantification of bound probe by dual beam flow cytometry …