Multicellular tumour spheroids were prepared from a total of 46 human brain tumour
biopsies by collagenase digestion and plating into agar coated flasks. Both primary …

The serum-free culture of multicellular tumor spheroids from two rat (BT4c, BT5c) and two
human (GaMg, D-54Mg) glioma cell lines is described. The spheroids were propagated in …

Human tumour cells from surgical material were grown as multicellular spheroids. In 16 out
of 20 culturesspheroids with a diameter of more than Z 5 0 p could be observed. In 6 out of …

✓ Tumor tissue from seven human gliomas was maintained in long-term agar overlay culture
as multicellular organotypic spheroids. Light microscopic and ultrastructural observation of …

A new technique, based on the growth of tumor cells in liquid media over an agar base, has
been developed for the formation and growth of multicellular tumor spheroids. All of the 11 …

Aggregates of human tumor cells are widely used in experimental studies on tumor
responses to treatment. Only a limited number of human tumor cell lines are capable of …

A poorly vascularized solid tumour can have many properties that complicate the situation
for drug treatment. The deficient blood vessel support will lead to regions consisting of well …

Multicellular tumour spheroids have become widely used as a 3-dimensional in vitro model
system in radiobiology and experimental chemotherapy (Sutherland & Durand, 1976; Yuhas …

The purpose of the meeting was to exchange information on spheroid culture methods and
to review the role of spheroids in cancer research. Techniques and results presented at this …

Growth curves for multicellular spheroids of 15 different tumor and normal cell lines were
characterized by doubling times which decreased with increasing growth until a stable …