On-site cellulase production and efficient saccharification of corn stover employing cbh2 overexpressing Trichoderma reesei with novel induction system

Y Li, X Zhang, L Xiong, MA Mehmood, X Zhao… - Bioresource …, 2017 - Elsevier
Although on-site cellulase production offers cost-effective saccharification of lignocellulosic
biomass, low enzyme titer is still a barrier for achieving robustness. In the present study, a …

Efficient preparation of soluble inducer for cellulase production and saccharification of corn stover using in-house generated crude enzymes

Y Li, P Zhang, D Zhu, B Yao, T Hasunuma… - Biochemical …, 2022 - Elsevier
Although high-level cellulase production by Trichoderma reesei from lignocellulosic
biomass in biorefinery has been widely studied, more efficient and economic hydrolysis is …

[HTML][HTML] Production of the versatile cellulase for cellulose bioconversion and cellulase inducer synthesis by genetic improvement of Trichoderma reesei

J Gao, Y Qian, Y Wang, Y Qu, Y Zhong - Biotechnology for biofuels, 2017 - Springer
Background The enzymes for efficient hydrolysis of lignocellulosic biomass are a major
factor in the development of an economically feasible cellulose bioconversion process. Up …

[HTML][HTML] Optimization of cellulolytic enzyme components through engineering Trichoderma reesei and on-site fermentation using the soluble inducer for cellulosic …

YH Li, XY Zhang, F Zhang, LC Peng, DB Zhang… - Biotechnology for …, 2018 - Springer
Background Cellulolytic enzymes produced by Trichoderma reesei are widely studied for
biomass bioconversion, and enzymatic components vary depending on different inducers. In …

Engineering Trichoderma reesei for Hyperproduction of Cellulases on Glucose to Efficiently Saccharify Pretreated Corncobs

F Zheng, R Yang, Y Cao, W Zhang, X Lv… - Journal of Agricultural …, 2020 - ACS Publications
The filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina) is widely used
as a cellulase producer in the industry. Herein, we describe the rational engineering of the …

Overproduction of cellulase by Trichoderma reesei RUT C30 through batch-feeding of synthesized low-cost sugar mixture

Y Li, C Liu, F Bai, X Zhao - Bioresource technology, 2016 - Elsevier
Cellulase is a prerequisite for the bioconversion of lignocellulosic biomass, but its high cost
presents the biggest challenge. In this article, low-cost mixture was produced from glucose …

[HTML][HTML] Production of highly efficient cellulase mixtures by genetically exploiting the potentials of Trichoderma reesei endogenous cellulases for hydrolysis of corncob …

Y Qian, L Zhong, J Gao, N Sun, Y Wang, G Sun… - Microbial Cell …, 2017 - Springer
Background Trichoderma reesei is one of the most important fungi utilized for cellulase
production. However, its cellulase system has been proven to be present in suboptimal ratio …

Cellulase production by recombinant Trichoderma reesei and its application in enzymatic hydrolysis of agricultural residues

H Fang, L Xia - Fuel, 2015 - Elsevier
Fed-batch fermentation was employed to improve the productivity of Trichoderma reesei
cellulase. The optimal conditions were fed-batch initiating time 48 h, lactose concentration …

Establishment of a highly efficient and low cost mixed cellulase system for bioconversion of corn stover by Trichoderma reesei and Aspergillus niger

L Shen, Y Su, Y Sun, G Wang, H Chen, X Yu… - Biocatalysis and …, 2021 - Elsevier
The high cost of cellulase production is one of the main obstacles for the conversion of
lignocellulose into high valued products. As a widely existing biomass resource, corn stover …

High activity cellulase production by recombinant Trichoderma reesei ZU-02 with the enhanced cellobiohydrolase production

H Fang, L Xia - Bioresource technology, 2013 - Elsevier
The cbh1 strong promoter was employed to over-express the cbh2 gene for enhancing
cellobiohydrolase (CBH) production in Trichoderma reesei because CBH II component has …