Background Embryonic development proceeds through finely tuned reprogramming of the
parental genomes to form a totipotent embryo. Cells within this embryo will then differentiate …

An open chromatin architecture devoid of compact chromatin is thought to be associated
with pluripotency in embryonic stem cells. Establishing this distinct epigenetic state may also …

In mammals, epigenetic reprogramming, the acquisition and loss of totipotency, and the first
cell fate decision all occur within a 3-d window after fertilization from the one-cell zygote to …

In mammals, epigenetic modifications are globally rearranged after fertilization, when
gametes fuse to form the embryo. While gametes carry special epigenetic signatures and a …

Epigenetic changes, including DNA methylation patterns, histone modifications and histone
variants, as well as chromatin remodelling play a fundamental role in the regulation of pre …

Over the last decade, our understanding of how the genome is packaged in three dimension
within the nucleus has grown considerably. This is largely due to advances in high …

Chromatin organization within the three-dimensional (3D) nuclear space is important for
proper gene expression and developmental programming. This organization is established …

Cis-regulatory elements regulate gene expression and lineage specification. However, the
potential regulation of cis-elements on mammalian embryogenesis remains largely …

Nuclear organization has emerged as a potential key regulator of genome function. During
development, the deployment of transcriptional programs must be tightly coordinated with …

This protocol presents a method to perform quantitative, single-cell in situ analyses of
protein expression to study lineage specification in mouse preimplantation embryos. The …