1 Introduction 1I Basm prmctples A Why detergents 9 B Choice of detergent C Kmetm
conslderatmns D Replacement of one detergent by another E Membrane reconsututmn F …

The mode by which four nonionic detergents interact with integral membrane proteins was
studied by relating detergent binding to characteristic detergent monolayer and micellar …

Detergents are indispensable in the isolation of integral membrane proteins from biological
membranes to study their intrinsic structural and functional properties. Solubilization …

The average hydrophobicity of a polypeptide segment is considered to be the most
important factor in the formation of transmembrane helices, and the partitioning of the most …

Binding of dodecyloctaethyleneglycol monoether (C12E3) and purified Triton X‐100 to
various integral membrane proteins was studied by chromatographic procedures. Binding …

III Measurement of membrane protem degradation A Chenucal labehng of cell surface
proteins B Metabolic labehng stuches followed by membrane isolation C Metabohc labehng …

Whereas some years ago most biologists were in substantial agreement as to what might be
the prevalent molecular organization of membranes, such consensus has now largely …

Membrane lipid composition can vary dramatically across the three domains of life and even
within single organisms. Here we review evidence that the lipid-exposed surfaces of …

Publisher Summary This chapter reviews the use of detergents for solubilization of
membranes and as a solvent medium for membrane proteins. It summarizes the properties …

The use of detergents for the structural study of membrane proteins is discussed with an
emphasis on practical issues relating to membrane solubilization, protein aggregation …