The cytoplasm of a living cell is a dynamic environment through which intracellular
components must move and mix. In motile, rapidly deforming cells such as human …

The ability of neutrophils to sense and move to sites of infection is essential for our defense
against pathogens. For motility, lamellipodium extension and stabilization are prerequisites …

We describe a technique for deconvolving the stochastic motion of particles from large-scale
fluid flow in a dynamic environment such as that found in living cells. The method leverages …

Phagocytic cells form the first line of defense in an organism, engulfing microbial pathogens.
Phagocytosis involves cell mechanical changes that are not yet well understood …

Neutrophils reach the sites of inflammation and infection in a timely manner by navigating
efficiently through mechanically complex interstitial spaces, following the guidance of …

We find that in contrast to strongly adherent, slow moving cells such as fibroblasts,
neutrophils exert contractile stresses largely in the rear of the cell (uropod) relative to the …

Highlights•Leukocytes have developed specific mechanisms to move in tissues.•Specific
cytoskeleton arrangements control cell migration in complex environments.•Fast leukocytes' …

Intracellular rheology is a useful probe of the mechanisms underlying spontaneous or
chemotactic locomotion and transcellular migration of leukocytes. We characterized regional …

Macrophage cell lines like J774 cells are ideal model systems for establishing the
biophysical foundations of autonomous deformation and motility of immune cells. To aid …

Fluorescence confocal microscopy was used to obtain three-dimensional (3-D) images of
human neutrophils migrating through a 3-D matrix of amniotic membrane with a temporal …