Target Choice and Orientation Preference of the Insertion Sequence IS903

WY Hu, KM Derbyshire - Journal of bacteriology, 1998 - Am Soc Microbiol
We have examined the targeting preference of the bacterial insertion element IS 903 by
determining the sites of insertion of a large number of transposition events into the 55-kb …

Insertion of transposon Tn7 into the Escherichia coli glmS transcriptional terminator

NJ Gay, VL Tybulewicz, JE Walker - Biochemical Journal, 1986 - portlandpress.com
The transposon Tn7 is unusual as it transposes at high frequencies from episomal elements
to a unique site in the Escherichia coli chromosome. This unique site is within a region of …

Atypical association of DDE transposition with conjugation specifies a new family of mobile elements

M Brochet, V Da Cunha, E Couvé… - Molecular …, 2009 - Wiley Online Library
We describe in Streptococcus agalactiae an atypical family of conjugative transposons
named TnGBSs which associates DDE transposition and conjugation. We present evidence …

[HTML][HTML] Tipping the balance between replicative and simple transposition

NP Tavakoli, KM Derbyshire - The EMBO Journal, 2001 - embopress.org
The bacterial insertion sequence IS903 has the unusual ability to transpose both
replicatively and non‐replicatively. The majority of products are simple insertions, while co …

Role of instability in the cis action of the insertion sequence IS903 transposase.

KM Derbyshire, M Kramer… - Proceedings of the …, 1990 - National Acad Sciences
An unusual subset of DNA-binding proteins, termed cis-acting proteins, has been shown to
act preferentially at their site of synthesis; the transposases of several bacterial insertion …

Insertion specificity and trans-activation of IS801

GY Richter, K Björklöf, M Romantschuk… - Molecular and General …, 1998 - Springer
The transposable element IS 801, isolated from plasmid pMMC7105 of Pseudomonas
syringae pv. phaseolicola, transposes in Escherichia coli to plasmid targets, expressing a …

Control of SXT integration and excision

V Burrus, MK Waldor - Journal of bacteriology, 2003 - Am Soc Microbiol
The Vibrio cholerae SXT element is a conjugative self-transmissible chromosomally
integrating element that encodes resistance to multiple antibiotics. SXT integrates in a site …

Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning of …

C Gawron-Burke, DB Clewell - Journal of bacteriology, 1984 - Am Soc Microbiol
The conjugative transposon Tn916 (15 kilobases), originally identified in Streptococcus
faecalis DS16, has been cloned as an intact element on the pBR322-derived vector pGL101 …

Is the IS1 transposase, InsAB', the only IS1-encoded protein required for efficient transposition?

JM Escoubas, D Lane, M Chandler - Journal of bacteriology, 1994 - Am Soc Microbiol
The transposase of the bacterial insertion sequence IS1 is normally expressed by inefficient
translational frameshifting between an upstream reading frame which itself specifies a …

Recognition of Escherichia coli attTn7 by transposon Tn7: lack of specific sequence requirements at the point of Tn7 insertion

E Gringauz, KA Orle, CS Waddell… - Journal of …, 1988 - Am Soc Microbiol
Transposon Tn7 inserts at high frequency into a specific site in the Escherichia coli
chromosome called attTn7. We show that the point of Tn7 insertion in attTn7 lies within the …