Insertion sequence IS50R, which encodes the transposase and an inhibitor of transposition
of the kanamycin-resistance transposon Tn5, increases the growth rate of E. coli K12 cells …

The bacterial transposon Tn7 is distinguished by its ability to recognize a specific site called
attTn7, and insert just downstream of the highly conserved chromosomal glmS gene. TnsD …

Excision from the donor DNA molecule is the first step in conjugative transposition of Tn916
and is followed by circularization of the transposon and its transfer to a new host. We have …

Bacterial conjugation, a mechanism of horizontal gene transfer, is the major means by which
antibiotic resistance spreads among bacteria (1, 2). Conjugative plasmids are transferred …

We have developed a strategy to rapidly construct DNA hybridization probes for the isolation
of genes disrupted by transposon Tn5 insertions. A single oligonucleotide complementary to …

Conjugation systems are present on many plasmids as well as on chromosomally integrated
elements. Conjugation, which is a major route by which bacteria exchange genetic material …

We have developed a series of shuttle vectors based on the conjugative transposon Tn916
that have been designed for the analysis of transcriptional regulation in Streptococcus …

Delivery vectors for mini-Tn10 transposons function in Bacillus subtilis (MA Petit, C. Bruand,
L. Janniére, and SD Ehrlich, J. Bacteriol. 172: 6736-6740, 1990). Using this system, we …

Hybrid plasmids containing the regulated meta-cleavage pathway operon of TOL plasmid
pWWO were mutagenized with transposon Tn1000 or Tn5. The resulting insertion mutant …

The bacterial transposon Tn7 is distinguished among mobile genetic elements by its
targeting abilities. Recently, we reported that Tn7 is able to selectively insert adjacent to …