D II. 1 Getting started: Expression and characterization of esterases 73 D II. 2 Enzymes for
the oxidation of primary alcohols to aldehydes in vivo 75 D II. 2.1 Unsuccessful substrate …

The majority of chemical transformations in metabolism are accelerated and controlled by a
large number of sophisticated enzymes, which were optimized by natural evolution to fulfill …

A synthetic cascade for the transformation of primary alcohols into polyhydroxylated
compounds in Escherichia coli, through the in situ preparation of cytotoxic aldehyde …

Enzymes are the most proficient catalysts we know and the number of processes that take
advantage of their enormous rate accelerations is constantly growing. The precision …

Advances in synthetic biology and metabolic engineering have enabled the construction of
novel biological routes to valuable chemicals using suitable microbial hosts. Aldehydes …

Metabolic engineering efforts have led to the development of bioprocesses implemented at
an industrial scale for the production of natural and non-natural chemicals. However, several …

De novo biocatalysts have been successfully generated by computational design and
subsequent experimental optimization. Here, we examined the evolutionary history of the …

Substrate specificity in the aldo-keto reductase (AKR) superfamily is determined by three
mobile loops positioned at the top of the canonical (α/β) 8-barrel structure. These loops have …

This review focuses on the directed evolution of aldolases with synthetically useful
properties. Directed evolution has been used to address a number of limitations associated …

We recently used a synthetic/bottom-up approach to establish the identity of the four
enzymes composing an engineered functional reversal of the β-oxidation cycle for fuel and …