It is now established that Plasmodium falciparum parasites found in any one area are highly
diverse and that individual hosts, vertebrate and insect, are often concurrently infected by …

The epidemiology of malaria results from the interactions of three gene pools--parasite,
human, and mosquito vector--with one another and with their environment. Methods are …

Many studies in molecular epidemiology of malaria require the researcher to enumerate or
to characterize multiple Plasmodium falciparum infections found concurrently in one carrier …

Publisher Summary This chapter focuses on those forms of genetic diversity that are clearly
understood at the deoxyribonucleic acid (DNA) level. The application of recombinant DNA …

We report on an analysis of the constraints of PCR typing of field Plasmodium falciparum
isolates by using a few highly polymorphic markers, MSA-1, MSA-2, TRAP, and CS. We …

We have analyzed 75 isolates of Plasmodium falciparum, collected in Venezuela during
both the dry (November) and rainy (May–July) seasons, with a range of genetic markers …

A method using DNA fingerprinting to distinguish Plasmodium falciparum isolates has been
developed using a clone, 7H8/6, isolated by screening a genomic expression library of the …

In the natural and untreated intermediate hosts, Plasmodium infections are maintained for
extended and often lifelong periods. Parasitemia generally reaches its highest level in the …

Laboratory studies of the protozoan parasite Plasmodium falciparum have been hampered
by difficulties in defining differences between isolates. We have developed a method based …

A multiplex PCR was designed for the differential diagnosis of the two parasite species by
targeting the 18S rRNA gene with a set of primer combinations, amplifying DNA fragments of …