Long‐term culture of human embryonic stem cells in feeder‐free conditions

ES Rosler, GJ Fisk, X Ares, J Irving… - … dynamics: an official …, 2004 - Wiley Online Library
We have demonstrated previously that human embryonic stem (hES) cells possess a
characteristic morphologic, antigenic, and molecular profile that can be used to assess the …

Properties of four human embryonic stem cell lines maintained in a feeder‐free culture system

MK Carpenter, ES Rosler, GJ Fisk… - … dynamics: an official …, 2004 - Wiley Online Library
Several laboratories have begun evaluating human ES (hES) cell lines; however, direct
comparisons between different hES cell lines have not been performed. We have …

Derivation, characterization, and differentiation of human embryonic stem cells

N Heins, MCO Englund, C Sjöblom, U Dahl… - Stem …, 2004 - academic.oup.com
The derivation of human embryonic stem (hES) cells establishes a new avenue to approach
many issues in human biology and medicine for the first time. To meet the increased …

Defined culture conditions of human embryonic stem cells

J Lu, R Hou, CJ Booth, SH Yang… - Proceedings of the …, 2006 - National Acad Sciences
Human embryonic stem cells (hESCs) are pluripotent cells that have the potential to
differentiate into any tissue in the human body; therefore, they are a valuable resource for …

Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to …

SN Brimble, X Zeng, DA Weiler, Y Luo… - Stem cells and …, 2004 - liebertpub.com
The number of human embryonic stem cell (hESC) lines available to federally funded US
researchers is currently limited. Thus, determining their basic characteristics and …

Characterization and differentiation of human embryonic stem cells

MK Carpenter, E Rosler, MS Rao - Cloning & Stem Cells, 2003 - liebertpub.com
Cell replacement therapies have been limited by the availability of sufficient quantities of
cells for transplantation. Human ES (hES) cell lines have recently been generated by …

[HTML][HTML] Differences between human and mouse embryonic stem cells

I Ginis, Y Luo, T Miura, S Thies, R Brandenberger… - Developmental …, 2004 - Elsevier
We compared gene expression profiles of mouse and human ES cells by
immunocytochemistry, RT-PCR, and membrane-based focused cDNA array analysis …

Multilineage differentiation from human embryonic stem cell lines

JS Odorico, DS Kaufman, JA Thomson - Stem cells, 2001 - academic.oup.com
Stem cells are unique cell populations with the ability to undergo both self-renewal and
differentiation. A wide variety of adult mammalian tissues harbors stem cells, yet “adult” stem …

Culture and characterization of human embryonic stem cells

JS Draper, HD Moore, LN Ruban… - Stem cells and …, 2004 - liebertpub.com
Human embryonic stem (ES) cells offer substantial opportunities for providing well-defined
differentiated cells for drug discovery, toxicology, and regenerative medicine, but the …

Distinct differentiation characteristics of individual human embryonic stem cell lines

M Mikkola, C Olsson, J Palgi, J Ustinov… - BMC Developmental …, 2006 - Springer
Background Individual differences between human embryonic stem cell (hESC) lines are
poorly understood. Here, we describe the derivation of five hESC lines (called FES 21, 22 …