The effect of nitrate and ammonium concentrations on growth and alkaloid accumulation of Atropa belladonna hairy roots

L Bensaddek, F Gillet, JEN Saucedo… - Journal of biotechnology, 2001 - Elsevier
The growth, the alkaloid production, as well as the scopolamine/hyoscyamine ratio of two
clones of belladonna hairy roots were studied. The effects of nitrate and ammonium …

Variation of alkaloid productivity among several clones of hairy roots and regenerated plants ofAtropa belladonna transformed withAgrobacterium rhizogenes 15834

T Aoki, H Matsumoto, Y Asako, Y Matsunaga… - Plant Cell Reports, 1997 - Springer
Hairy root cultures of Atropabelladonna were established by transformation with
Agrobacterium rhizogenes 15834. Five clones of them were employed to study the …

Characteristics of growth and tropane alkaloid synthesis in Atropa belladonna roots transformed by Agrobacterium rhizogenes

JM Sharp, PM Doran - Journal of Biotechnology, 1990 - Elsevier
Growth of Atropa belladonna hairy roots in shake flasks followed first-order kinetics. The
specific growth rate was 0.28 d− 1, corresponding to a doubling time of 60 h. In airlift …

Studies on the optimization of growth and indole alkaloid production by hairy root cultures of Catharanthus roseus

L Toivonen, M Ojala… - Biotechnology and …, 1991 - Wiley Online Library
Catharanthus roseus hairy root cultures, genetically transformed with Agrobacterium
rhizogenes, produce a wide variety of indole alkaloids. The effect of sucrose, phosphate …

Differential production of tropane alkaloids in hairy roots and in vitro cultured two accessions of Atropa belladonna L. under nitrate treatments

NA Chashmi, M Sharifi, F Karimi… - … für Naturforschung C, 2010 - degruyter.com
Plants are a potential source of a large number of valuable secondary metabolites. In vitro
cultures are being considered as an alternative to agricultural processes for studying …

Tropane alkaloid production by hairy roots of Atropa belladonna obtained after transformation with Agrobacteriumrhizogenes 15834 and Agrobacterium tumefaciens …

V Bonhomme, D Laurain-Mattar, J Lacoux… - Journal of …, 2000 - Elsevier
Atropa belladonna leaf disks were infected by a wild strain Agrobacterium rhizogenes 15834
harboring the Ri-TL-DNA and by a disarmed Agrobacterium tumefaciens strain harboring a …

Alkaloid production by hairy root cultures in Atropa belladonna

H Kamada, N Okamura, M Satake, H Harada… - Plant cell reports, 1986 - Springer
Hairy roots were induced by inoculation of stems of sterile plants of Atropa belladonna with
Agrobacterium rhizogenes. The axenic culture of the hairy roots isolated from the stems …

Effects of chemicals on alkaloid production by transformed roots of belladonna

KT Lee, T Yamakawa, T Kodama, K Shimomura - Phytochemistry, 1998 - Elsevier
The growth and tropane content in transformed root cultures of Atropa belladonna (strain
M8) grown in 300 ml flasks were studied. After one month in culture, the biomass of the …

Production of tropane alkaloids by hairy root cultures of Duboisia leichhardtii transformed by Agrobacterium rhizogenes

Y Mano, H Ohkawa, Y Yamada - Plant Science, 1989 - Elsevier
Hairy roots were induced by inoculating leaf disks and stem segments of Duboisia
leichhardtii F. Muell with Agrobacterium rhizogenes strains 15834 and A4. Forty-five hairy …

Characteristics of growth and tropane alkaloid production in Hyoscyamus albus hairy roots transformed with Agrobacterium rhizogenes A4

P Christen, T Aoki, K Shimomura - Plant Cell Reports, 1992 - Springer
Hairy root culture of Hyoscyamus albus was established by transformation with
Agrobacterium rhizogenes strain A4. The growth and production of five tropane alkaloids …