Liquid chromatography–mass spectrometry-based metabolomics studies require highly
selective and efficient chromatographic techniques. Typically employed reversed-phase …

In the present work two different approaches, a semi-quantitative and a Derringer function
approach, were developed to assist column selection for method development in targeted …

Profiling of body fluids is crucial for monitoring and discovering metabolic markers of health
and disease and for providing insights into human physiology. Since human urine and …

Hydrophilic interaction chromatography (HILIC) is an emerging separation mode of liquid
chromatography (LC). Using highly hydrophilic stationary phases capable of retaining …

Hydrophilic interaction chromatography (HILIC) was introduced more than two decades ago
and has garnered much attention. Characterized by a hydrophilic stationary phase used in …

Although it is common in untargeted metabolomics to apply reversed-phase liquid
chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) methods …

Hydrophilic metabolites are essential for all biological systems with multiple functions and
their quantitative analysis forms an important part of metabolomics. However, poor retention …

Metabolomics involves qualitative and/or quantitative analysis of hundreds of metabolites in
a complex sample. As most of the metabolites are polar in nature (for example, amino acids …

This work aims at evaluating the relevance and versatility of liquid chromatography coupled
to high resolution mass spectrometry (LC/HRMS) for performing a qualitative and …

Abstract Evaluation of the chromatographic separation in metabolomics studies has
primarily been done using preselected sets of standards or by counting the number of …