A series of dipeptide derivatives of Rhodamine, each containing an arginine residue in the
P1 position and one of ten representative benzyloxycarbonyl (Cbz)-blocked amino acids in …

A new fluorogenic substrate for serine proteinases, bis (N-benzyloxycarbonyl-L-
argininamido) Rhodamine [(Cbz-Arg-NH) 2-Rhodamine], was synthesized, purified and …

Publisher Summary This chapter focuses on the thioester peptide chloromethyl ketones that
are the reagents for active site-selective labeling of serine proteinases with spectroscopic …

p-Aminobenzamidine is weakly fluorescent in neutral aqueous buffer, with excitation and
emission maxima at 293 and 376 nm, respectively. Binding to trypsin results in a blue shift of …

D. Campbell Livingston, John R. Brocklehurst, John F. Cannon, 1 Steven P. Leytus, John A.
Wehrly, 8 Stuart W. Peltz, Gary A. Peltz, 1 and Walter F. Mangel** abstract: The molecule 3' …

The action of serine (and cysteine) proteases on peptide esters proceeds, as a
generalization, orders of magnitude faster than the corresponding enzymatic hydrolysis of …

Revised Manuscript Received April 4, 1988 abstract: The feasibility of a new approach to
incorporation of spectroscopic probes into the active sites of certain serine proteases has …

Two active-site titrants of serine proteases, fluorescein mono-p-
guanidinobenzoatehydrochloride (FMGB-HC1) and fluorescein di-p-guanidinobenzoate …

Alkyl isocyanates as active-site-specific reagents for serine protease. Identification of the
active-site serine as the site of Page 1 ALKYL ISOCYANATE-PROTEASE REACTION Alkyl …

The serine proteases comprise a large group of enzymes which is distinguished by the
reactivity of a serine residue in the active site. 1 A common test for these enzymes is the …