Enhanced scale and scope of genome engineering and regulation using CRISPR/Cas in Saccharomyces cerevisiae

M Deaner, HS Alper - FEMS Yeast Research, 2019 - academic.oup.com
Although only 6 years old, the CRISPR system has blossomed into a tool for rapid, on-
demand genome engineering and gene regulation in Saccharomyces cerevisiae. In this …

[HTML][HTML] Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects

H Raschmanová, A Weninger, A Glieder, K Kovar… - Biotechnology …, 2018 - Elsevier
Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome
engineering, while also changing the speed and efficiency of metabolic engineering in …

[HTML][HTML] Focus: Genome Editing: Yeast Still a Beast: Diverse Applications of CRISPR/Cas Editing Technology in S. cerevisiae

RM Giersch, GC Finnigan - The Yale Journal of Biology and …, 2017 - ncbi.nlm.nih.gov
The recent discovery and use of CRISPR/Cas9 gene editing technology has provided new
opportunities for scientific research in many fields of study including agriculture, genetic …

Tips, Tricks, and Potential Pitfalls of CRISPR Genome Editing in Saccharomyces cerevisiae

JS Antony, JM Hinz, JJ Wyrick - Frontiers in Bioengineering and …, 2022 - frontiersin.org
The versatility of clustered regularly interspaced short palindromic repeat (CRISPR)-
associated (Cas) genome editing makes it a popular tool for many research and …

CRISPR‐enabled tools for engineering microbial genomes and phenotypes

K Tarasava, EJ Oh, CA Eckert, RT Gill - Biotechnology Journal, 2018 - Wiley Online Library
In recent years CRISPR‐Cas technologies have revolutionized microbial engineering
approaches. Genome editing and non‐editing applications of various CRISPR‐Cas systems …

CRISPR-Cas systems for editing, regulating and targeting genomes

JD Sander, JK Joung - Nature biotechnology, 2014 - nature.com
Targeted genome editing using engineered nucleases has rapidly gone from being a niche
technology to a mainstream method used by many biological researchers. This widespread …

Development and expansion of the CRISPR/Cas9 toolboxes for powerful genome engineering in yeast

G Cai, Z Lin, S Shi - Enzyme and Microbial Technology, 2022 - Elsevier
Yeasts represent a group of the microorganisms most frequently seen in biotechnology.
Recently, the class 2 type II CRISPR system (CRISPR/Cas9) has become the principal …

[HTML][HTML] CRISPR-Cas systems for genome editing, regulation and targeting

JD Sander, JK Joung - Nature biotechnology, 2014 - ncbi.nlm.nih.gov
Targeted genome editing using engineered nucleases has rapidly transformed from a niche
technology to a mainstream method used by many biological researchers. This widespread …

CRISPR-Cas guides the future of genetic engineering

GJ Knott, JA Doudna - Science, 2018 - science.org
The diversity, modularity, and efficacy of CRISPR-Cas systems are driving a
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …

Expanding the CRISPR/Cas9 Toolbox for Gene Engineering in S. cerevisiae

O Levi, Y Arava - Current microbiology, 2020 - Springer
The yeast S. cerevisiae serves as a model organism for many decades. Numerous
molecular tools have been developed throughout the years to engineer its genome …