Immunoblot analysis of Dermatophagoides farinae antigen.

K Nakanishi, K Shimokata - Annals of allergy, 1990 - europepmc.org
We isolated the allergenic components of Dermatophagoides farinae (D. farinae) body
extracts and determined the frequencies and specificities of IgE, IgG, and IgG4 antibodies …

Isolation and characterization of the main allergen of Dermatophagoides farinae by monoclonal antibodies that recognize IgE related epitopes

V Ley, JC Sáiz, J Carreira - Molecular immunology, 1986 - Elsevier
Mouse monoclonal antibodies (MAbs) with different specificities against Dermatophagoides
farinae (D. farinae) extract have been obtained. Fifteen of these antibodies reacted with …

Isolation and characterization of two allergens from Dermatophagoides farinae

H Yasueda, H Mita, Y Yui, T Shida - International archives of allergy and …, 1986 - karger.com
Two purified allergens, designated as DF1 and DF2, were isolated from the extract of the
whole culture of Dermatophagoides farinae by a combination of ammonium sulfate …

Antigens and allergens in Dermatophagoïdes farinae mite. I. Immunochemical and physicochemical study of two allergenic fractions from a partially-purified …

J Le Mao, JP Dandeu, J Rabillon, M Lux, B David - Immunology, 1981 - ncbi.nlm.nih.gov
The fractionation of a partially-purified extract of Dermatophagoïdes farinae mite culture has
been undertaken by gel filtration. Two fractions were isolated. One, P25, is a protein-rich …

Identification of a novel allergen molecule from Dermatophagoides by monoclonal antibodies

V Ley, P de la Hoz, AL Corbi, F Sanchez-Madrid… - Immunology letters, 1985 - Elsevier
Six different monoclonal antibodies (MAb) have been obtained which bind to components
contained in extracts from Dermatophagoides. One out of the six MAb recognized molecules …

IgE Responsiveness to Dermatophagoides farinae in Asthma and Atopic Dermatitis

E Noguchi, M Shibasaki - International archives of allergy and …, 1997 - karger.com
Using Western blotting and RAST, this study examined IgE reactivities to Dermatophagoides
farinae (Df) extract in three groups of children with either asthma, atopic dermatitis (AD) or a …

Allergenic activity of soluble fractions ofDermatophagoides farinae

NL Woodiel, SE Bennett, RP Hornsby… - Journal of Allergy and …, 1974 - Elsevier
D. farinae mite fluids were separated by polyacrylamide gel electrophoresis. Four prominent
bands were selected from stained gels, and corresponding gel regions eluted from …

Identification of Main Allergens from Dermatophagoidesfarinae and Their Properties under Native and Dissociating Conditions

F de la Hoz, J Carreira - International Archives of Allergy and …, 1986 - karger.com
Dermatophagoides farinae extract has been fractionated by preparative isoelectric focusing.
Fraction analysis showed that the total allergenic potency was distributed along pi values …

Measurement of IgG, IgA and IgE antibodies to Dermatophagoides pteronyssinus by antigen-binding assay, using a partially purified fraction of mite extract (F4P1).

MD Chapman, TA Platts-Mills - clinical and Experimental …, 1978 - ncbi.nlm.nih.gov
An extract of Dermatophagoides pteronyssinus culture has been fractionated by
chromatography on Sephadex G-100 and Pevikon block electrophoresis to obtain a partially …

Anaphylaxis after ingestion of beignets contaminated with Dermatophagoides farinae

AM Erben, JL Rodriguez, J McCullough… - Journal of allergy and …, 1993 - Elsevier
A 48-year-old man was evaluated for anaphylaxis associated with ingestion of beignets
prepared from a commercial mix. Microscopic examination of the patient's beignet mix …