Contribution Ratios of amyA, amyB, amyC Genes to High-Level α-Amylase Expression in Aspergillus oryzae

T Nemoto, J Maruyama… - Bioscience, biotechnology …, 2012 - academic.oup.com
Aspergillus oryzae strains express α-amylases abundantly, and the genome reference strain
RIB40 has three α-amylase genes (amyA, amyB, and amyC). However, there is no …

Molecular Cloning and Characterization of a Transcriptional Activator Gene, amyR, Involved in the Amylolytic Gene Expression in Aspergillus oryzae

K Gomi, T Akeno, T Minetoki, K Ozeki… - Bioscience …, 2000 - academic.oup.com
A gene, designated amyR, coding for a transcriptional activator involved in amylolytic gene
expression has been cloned from Aspergillus oryzae by screening for a clone that enabled …

Insertion analysis of putative functional elements in the promoter region of the Aspergillus oryzae Taka-amylase A gene (amyB) using a heterologous Aspergillus …

Y Kanemori, K Gomi, K Kitamoto… - Bioscience …, 1999 - jstage.jst.go.jp
抄録 Expression of the Taka-amylase A gene (amyB) of Aspergillus oryzae is induced by
starch or maltose. The A. oryzae amyB gene promoter contains three highly conserved …

Improvement of Heterologous Protein Production in Aspergillus oryzae by RNA Interference with α-Amylase Genes

T Nemoto, J Maruyama… - Bioscience, biotechnology …, 2009 - academic.oup.com
Aspergillus oryzae RIB40 has three α-amylase genes (amyA, amyB, and amyC), and
secretes α-amylase abundantly. However, large amounts of endogenous secretory proteins …

Aspergillus oryzae S2 AmyA amylase expression in Pichia pastoris: production, purification and novel properties

S Trabelsi, M Sahnoun, F Elgharbi, R Ameri… - Molecular biology …, 2019 - Springer
A synthetic cDNA-AmyA gene was cloned and successfully expressed in Pichia pastoris as
a His-tagged enzyme under the methanol inducible AOX1 promoter. High level of …

Characteristic Expression of Three Amylase-encoding Genes, agdA, amyB, and glaA in Aspergillus oryzae Transformants Containing Multiple Copies of the agdA …

T Minetoki, K Gomi, K Kitamoto… - Bioscience …, 1995 - academic.oup.com
In Aspergillus oryzae wild-type strains, the expression of the agdA gene encoding α-
glucosidase (AGL) is induced by maltose at the transcriptional level in a similar manner to …

Cloning, characterization, and expression of two α-amylase genes from Aspergillus niger var. awamori

DR Korman, FT Bayliss, CC Barnett, CL Carmona… - Current genetics, 1990 - Springer
Using synthetic oligonucleotide probes, we cloned genomic DNA sequences encoding an α-
amylase gene from Aspergillus niger var. awamori (A. awamori) on a 5.8 kb Eco RI fragment …

Improvement of promoter activity by the introduction of multiple copies of the conserved region III sequence, involved in the efficient expression of Aspergillus oryzae …

T Minetoki, C Kumagai, K Gomi, K Kitamoto… - Applied microbiology …, 1998 - Springer
The role of the conserved sequence region III in the promoter regions of the amylase-
encoding genes amyB, glaA and agdA of Aspergillus oryzae was examined. Introduction of …

Construction of a shuttle vector for heterologous expression of a novel fungal α-amylase gene in Aspergillus oryzae

Y Yin, Y Mao, X Yin, B Gao, D Wei - Journal of Microbiology and …, 2015 - koreascience.kr
The filamentous fungus Aspergillus oryzae is a well-known expression host used to express
homologous and heterologous proteins in a number of industrial applications. To facilitate …

Expression of Aspergillus oryzae α-amylase gene in Saccharomyces cerevisiae

F Randez-Gil, P Sanz - FEMS microbiology letters, 1993 - academic.oup.com
A fragment containing the full length cDNA from Aspergillus oryzae α-amylase has been
amplified by PCR using specific synthetic oligonucleotides. The amplified cDNA was …