Background Genome sequencing yields the sequence of many short snippets of DNA
(reads) from a genome. Genome assembly attempts to reconstruct the original genome from …

Third generation single molecule sequencing technology is poised to revolutionize
genomics by enabling the sequencing of long, individual molecules of DNA and RNA. These …

Motivation: Genome assemblies generated with next-generation sequencing (NGS) reads
usually contain a number of gaps. Several tools have recently been developed to close the …

Motivation: No individual assembly algorithm addresses all the known limitations of
assembling short-length sequences. Overall reduced sequence contig length is the major …

Long-read single-molecule sequencing has revolutionized de novo genome assembly and
enabled the automated reconstruction of reference-quality genomes. It has also been widely …

Genome assembly depends critically on read length. Two recent technologies, from Pacific
Biosciences (PacBio) and Oxford Nanopore, produce read lengths> 20 kb, which yield de …

Despite the rapidly increasing number of sequenced and re-sequenced genomes, many
issues regarding the computational assembly of large-scale sequencing data have remain …

Accurate genome assembly is hampered by repetitive regions. Although long single
molecule sequencing reads are better able to resolve genomic repeats than short-read data …

Background Generating high-quality de novo genome assemblies is foundational to the
genomics study of model and non-model organisms. In recent years, long-read sequencing …

Assembly of a high-quality genome is important for downstream comparative and functional
genomic studies. However, most tools for genome assembly assessment only give …