Solubilization of inclusion body proteins using low and very low concentrations of chemicals: implications of novel combined chemical treatment designs in …
A Mohammadian, H Kaghazian… - Journal of Chemical …, 2018 - Wiley Online Library
BACKGROUND Solubilization of inclusion body (IB) proteins by conventional methods (ie
high concentrations of denaturants such as chaotropes) is a challenging process due to …
high concentrations of denaturants such as chaotropes) is a challenging process due to …
[HTML][HTML] Nonclassical inclusion bodies in Escherichia coli
Š Peternel, M Bele, V Gaberc-Porekar, V Menart - Microbial Cell Factories, 2006 - Springer
Background Formation of inclusion bodies (IBs) during over-expression of recombinant
proteins in Escherichia coli is of common occurrence. The target protein inside inclusion …
proteins in Escherichia coli is of common occurrence. The target protein inside inclusion …
Single pH buffer refolding screen for protein from inclusion bodies
We previously reported the set up of an automated test for screening the refolding of
recombinant proteins expressed as inclusion bodies in Escherichia coli [1]. The screen used …
recombinant proteins expressed as inclusion bodies in Escherichia coli [1]. The screen used …
Inclusion body anatomy and functioning of chaperone-mediated in vivo inclusion body disassembly during high-level recombinant protein production in Escherichia …
U Rinas, F Hoffmann, E Betiku, D Estapé… - Journal of biotechnology, 2007 - Elsevier
During production in recombinant Escherichia coli, the human basic fibroblast growth factor
(hFGF-2) partly aggregates into stable cytoplasmic inclusion bodies. These inclusion bodies …
(hFGF-2) partly aggregates into stable cytoplasmic inclusion bodies. These inclusion bodies …
[HTML][HTML] Learning about protein solubility from bacterial inclusion bodies
M Martínez-Alonso, N González-Montalbán… - Microbial cell …, 2009 - Springer
The progressive solving of the conformation of aggregated proteins and the conceptual
understanding of the biology of inclusion bodies in recombinant bacteria is providing …
understanding of the biology of inclusion bodies in recombinant bacteria is providing …
Protein folding in vivo and renaturation of recombinant proteins from inclusion bodies
AD Guise, SM West, JB Chaudhuri - Molecular biotechnology, 1996 - Springer
Eukaryotic proteins expressed in Escherichia coli often accumulate within the cell as
insoluble protein aggregates or inclusion bodies. The recovery of structure and activity from …
insoluble protein aggregates or inclusion bodies. The recovery of structure and activity from …
The economics of inclusion body processing
GH Lee, D Cooney, APJ Middelberg… - Bioprocess and biosystems …, 2006 - Springer
Many recombinant proteins are often over-expressed in host cells, such as Escherichia coli,
and are found as insoluble and inactive protein aggregates known as inclusion bodies (IBs) …
and are found as insoluble and inactive protein aggregates known as inclusion bodies (IBs) …
[HTML][HTML] Engineering inclusion bodies for non denaturing extraction of functional proteins
Š Peternel, J Grdadolnik, V Gaberc-Porekar… - Microbial cell …, 2008 - Springer
Background For a long time IBs were considered to be inactive deposits of accumulated
target proteins. In our previous studies, we discovered IBs containing a high percentage of …
target proteins. In our previous studies, we discovered IBs containing a high percentage of …
Ensemble modeling and intracellular aggregation of an engineered immunoglobulin-like domain
K Trainor, Z Gingras, C Shillingford, H Malakian… - Journal of molecular …, 2016 - Elsevier
The production of recombinant proteins in Escherichia coli frequently results in the formation
of insoluble protein aggregates called inclusion bodies (IBs). The determinants of IB …
of insoluble protein aggregates called inclusion bodies (IBs). The determinants of IB …