Simultaneous multianalyte ELISA performed on a microarray platform

R Wiese, Y Belosludtsev, T Powdrill… - Clinical …, 2001 - academic.oup.com
Background: A logical progression of the widely used microtiter plate ELISA is toward a
protein array format that allows simultaneous detection of multiple analytes at multiple array …

High-throughput genomic and proteomic analysis using microarray technology

JX Huang, D Mehrens, R Wiese, S Lee, SW Tam… - 2001 - academic.oup.com
Background: High-density microarrays are ideally suited for analyzing thousands of genes
against a small number of samples. The next step in the discovery process is to take the …

A 1024-sample serum analyzer chip for cancer diagnostics

JL Garcia-Cordero, SJ Maerkl - Lab on a Chip, 2014 - pubs.rsc.org
We present a platform that combines microarrays and microfluidic techniques to measure
four protein biomarkers in 1024 serum samples for a total of 4096 assays per device …

[HTML][HTML] Detection and quantification of protein biomarkers from fewer than 10 cells

S Nettikadan, K Radke, J Johnson, J Xu… - Molecular & Cellular …, 2006 - ASBMB
The use of antibody microarrays continues to grow rapidly due to the recent advances in
proteomics and automation and the opportunity this combination creates for high throughput …

Automated multiplexed ECL immunoarrays for cancer biomarker proteins

K Kadimisetty, S Malla, NP Sardesai, AA Joshi… - Analytical …, 2015 - ACS Publications
Point-of-care diagnostics based on multiplexed protein measurements face challenges of
simple, automated, low-cost, and high-throughput operation with high sensitivity. Herein, we …

Fifth-generation digital immunoassay for prostate-specific antigen by single molecule array technology

DH Wilson, DW Hanlon, GK Provuncher… - Clinical …, 2011 - academic.oup.com
BACKGROUND Measurement of prostate-specific antigen (PSA) in prostate cancer patients
following radical prostatectomy (RP) has been hindered by the limit of quantification of …

Diagnostic challenges for multiplexed protein microarrays

SR Master, C Bierl, LJ Kricka - Drug discovery today, 2006 - Elsevier
Multiplexed protein analysis using planar microarrays or microbeads is growing in popularity
for simultaneous assays of antibodies, cytokines, allergens, drugs and hormones. However …

Prostate cancer diagnosis in the clinic using an 8-protein biomarker panel

AL Jones, L Dhanapala, TA Baldo… - Analytical …, 2020 - ACS Publications
The inability to distinguish aggressive from indolent prostate cancer is a longstanding
clinical problem. Prostate specific antigen (PSA) tests and digital rectal exams cannot …

A comparability study of the emerging protein array platforms with established ELISA procedures

S Pang, J Smith, D Onley, J Reeve, M Walker… - Journal of immunological …, 2005 - Elsevier
Recent developments in microarray technology have made it possible to perform
immunoassays in a multiplexed format. This ability is highly desirable given the potential for …

Multiplexing molecular diagnostics and immunoassays using emerging microarray technologies

MM Ling, C Ricks, P Lea - Expert review of molecular diagnostics, 2007 - Taylor & Francis
Enzyme-linked immunosorbent assays (ELISA) are frequently used for quantitative
measurement of the presence of protein, for single-analyte testing, in a sample. The …