Developing an efficient transformation system is vital in genetically engineering recalcitrant
crops, particularly trees. Here, we outline an Agrobacterium tumefaciens-based stable plant …

Although several protocols for genetic transformation of citrus have been published, it is
highly desirable to further improve its efficiency. Here we report treatments of Agrobacterium …

Since the initial reports on production of transgenic Citrus via Agrobacterium-mediated
transformation, significant progress has been made, and many steps of this procedure using …

A procedure for increased efficiency of production of transgenic citrus plants was developed
by extending the exposure of the explants to the selection agent and by grafting in vitro the …

Mature tissue protocol for production of transgenic Citrus plants via Agrobacterium-mediated
transformation uses explants derived from branches of mature, fruit-bearing trees. Through …

Citrus are among the most prevailing fruit crops produced worldwide. The implementation of
effective and reliable breeding programs is essential for coping with the increasing demands …

Highly efficient Agrobacterium-mediated transformation of trifoliate orange (Poncirus
trifoliata (L.) Raf.) was achieved via indirect shoot organogenesis. Stable transformants were …

Transgenic Mexican lime (Citrus aurantifolia Swingle) was produced through two explant
sources, each using systems previously optimized for each source. One used epicotyls …

Citrus is one important fruit crop in China and worldwide. Genetic transformation with more
recently cloned target genes is an effective alternative for citrus improvement. In the past …

An improved protocol for genetic transformation of juvenile explants of Carrizo (Citrus
sinensis Osb.× Poncirus trifoliata L. Raf.), Duncan (Citrus paradisi Macf.), Hamlin (Citrus …