Intrasubunit homogeneity in heterogeneous IgM antibodies to the DNP moiety derived from a murine hybridoma cell line

R Giles, D Klapper, LW Clem - Molecular Immunology, 1982 - Elsevier
A hybridoma line secreting 19S IgM antibodies reactive with the DNP moiety and designated
14PAF was derived from a fusion of murine spleen cells with the myeloma line P3-X63-Ag8 …

Intramolecular heterogeneity of ligand binding by two IgM antibodies derived from murine hybridomas

RC Giles, DG Klapper, LW Clem - Molecular immunology, 1983 - Elsevier
The ligand binding properties of two murine anti-DNP IgM hybridoma antibodies were
analyzed. These IgM antibodies, designated NP3-17 C1-20 and SP2/0 I-64 C1–12, each …

Ligand binding by murine IgM antibodies: intramolecular heterogeneity exists in certain, but not all, cases

D Pascual, LW Clem - Molecular immunology, 1988 - Elsevier
The ligand binding properties of eight hybridoma-derived murine anti-DNP IgM (ϰ)
antibodies were analysed by equilibrium dialysis. Four of these proteins exhibited the …

Heterologous recombination between mouse myeloma 315 heavy chains and rabbit antibody light chains: structural and functional properties of the hybrid molecules

D Hoessli, J Olander, JR Little - The Journal of Immunology, 1974 - journals.aai.org
Abstract 7S recombinant molecules have been obtained from the heavy (H) chains of mouse
myeloma protein 315 (which binds 2, 4 dinitrophenyl (DNP) ligands) and the light (L) chains …

Structural analysis of the antibody combining site

D Givol - Contemporary Topics in Molecular Immunology, 1973 - Springer
The central problem of immunochemistry is to find the structural basis for antibody specificity.
In view of the very wide range of different antigenic determinants and the overall similar …

A screening method to detect clonal secretion of DNP‐specific antibody

GL Waring - Journal of Cellular Physiology, 1975 - Wiley Online Library
Spontaneous variants of the IgA immunoglobulin secreting mouse myeloma, S194–2, were
isolated by cloning the line on soft agar and screening for the loss of secreted S194 …

Structural studies on DNP binding antibodies

R Leatherbarrow - 1983 - ora.ox.ac.uk
This thesis is concerned with structural aspects of the recognition and effector functions of
antibody molecules. The recognition process is investigated in the dinitrophenyl (DNP) …

Subunit structure and number of combining sites of the immunoglobulin A myeloma protein produced by mouse plasmacytoma MOPC-315

BJ Underdown, ES Simms, HN Eisen - Biochemistry, 1971 - ACS Publications
Brian J. Underdown, f Ernest S. Simms, and HermanN. Eiseni abstract: The myeloma protein
produced by mouse plasma-cytoma MOPC-315 was studied with respect to its subunit …

Combining sites of anti-2, 4-dinitrophenyl antibodies

HN EISEN - Progress in Immunology, 1971 - Elsevier
Publisher Summary This chapter describes the combining sites of anti-2, 4-dinitrophenyl
(DNP) antibodies. The anti-DNP antibodies formed against dinitrophenylated antigens …

Antibodies to DNP, DNP—glycylglycylglycine and DNP—p-aminobenzoylglutamate

ME Woehler, LE Cannon, PD Clark, RE Lovins - Immunology, 1974 - ncbi.nlm.nih.gov
The antibodies to a series of three dinitrophenyl (DNP) derivatives of increasing site-filling
capacity (DNP; DNP—glycylglycylglycine and DNP—p-aminobenzolylglutamate) have been …