Long noncoding RNAs (lncRNAs) are crucial factors during plant development and
environmental responses. To build an accurate atlas of lncRNAs in the diploid cotton …

In plants, nitrogen is the most important nutritional factor limiting the yield of cultivated crops.
Since nitrogen is essential for synthesis of nucleotides, amino acids and proteins, studies on …

The mapping of functional genes plays an important role in studies of genome structure,
function, and evolution, as well as allowing gene cloning and marker-assisted selection to …

Verticillium wilt caused by the soil-borne fungus Verticillium dahliae, is a devastating
disease in cotton. To get more candidate genes related to wilt resistance, a normalized, full …

Gossypium herbaceum is a species of cotton native to Africa and Asia that is one of the 2
domesticated diploids. Together with its sister-species G. arboreum, these A-genome taxa …

The high-quality reference-grade genome for Gossupium tomentosum can greatly promote
the progress in biological research and introgression breeding for the mainly cultivated …

Reverse transcription-qPCR (RT-qPCR) has become a popular method for gene expression
studies. Its results require data normalization by housekeeping genes. No single gene is …

Background Advances in genome sequencing technology, particularly restriction-site
associated DNA sequence (RAD-seq) and whole-genome resequencing, have greatly aided …

qRT-PCR is a common and key technical means to study gene expression in biological
research. However, reliability and accuracy of quantification by qRT-PCR is entirely …

Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular
technique for investigating gene expression levels in plants. Based on the Minimum …