Unbiased quantitative analysis of macroscopic biological samples demands fast imaging
systems capable of maintaining high resolution across large volumes. Here we introduce …

Whole-brain imaging has become an increasingly important approach to investigate neural
structures, such as somata distribution, dendritic morphology, and axonal projection …

We introduce a cost-effective and easily implementable scan unit that converts any camera-
based microscope with optical sectioning capability into a multi-angle projection imaging …

Here, we describe an optimized and detailed protocol for block-face serial microscopy
tomography (FAST). FAST enables high-speed serial section fluorescence imaging of fixed …

A key challenge when imaging living cells is how to noninvasively extract the most
spatiotemporal information possible. Unlike popular wide-field and confocal methods, plane …

Image scanning microscopy (ISM) can improve the effective spatial resolution of confocal
microscopy to its theoretical limit. However, current implementations are not robust or …

In fluorescence microscopy, the serial acquisition of two-dimensional images to form a three-
dimensional (3D) volume limits the maximum imaging speed. This is particularly evident …

Non-uniform illumination limits quantitative analyses of fluorescence imaging techniques. In
particular, single molecule localization microscopy (SMLM) relies on high irradiances, but …

Imaging fast cellular dynamics across large specimens requires high resolution in all
dimensions, high imaging speeds, good physical coverage and low photo-damage. To meet …

In optical microscopy, the slow axial scanning rate of the objective or the sample has
traditionally limited the speed of volumetric imaging. Recently, by conjugating either a …