Effects of culture media and inhibitors on biology of porcine early embryonic development in vitro

H Wang, N Rodriguez-Osorio, JM Feugang, SY Jung… - Livestock Science, 2009 - Elsevier
In vitro-production of porcine embryos is crucial for biomedical and agricultural research,
however, current culture systems for porcine embryos are sub-optimal, and the …

Development and viability of in vitro derived porcine blastocysts cultured in NCSU23 and G1. 2/G2. 2 sequential medium

JE Swain, CL Bormann, RL Krisher - Theriogenology, 2001 - Elsevier
Porcine embryo development in vitro is relatively inefficient compared to other domestic
species. Currently, a single culture medium (NCSU23) is the standard for porcine in vitro …

Addition of macromolecules to PZM-3 culture medium on the development and hatching of in vitro porcine embryos

JY Kim, SB Kim, MC Park, H Park, YS Park… - … journal of animal …, 2007 - koreascience.kr
In this study, we conducted various experiments in order to develop enhanced cultural
conditions for in vitro-produced porcine embryos. All embryos were produced by in vitro …

Effect of culture media on porcine embryos produced by in vitro fertilization or parthenogenetic activation after oocyte maturation with cycloheximide

MG Marques, AB Nascimento, RP da Costa Gerger… - Zygote, 2011 - cambridge.org
This study evaluated the effects of reversible meiotic inhibition and different culture media
(PZM3 or NCSU23) on production of porcine embryos by either in vitro fertilization (IVF) or …

155 QUALITY OF PORCINE EMBRYO PRODUCED IN TWO EMBRYO CULTURE MEDIA AS ASSESSED BY TOTAL CELL NUMBER AND TIME COURSE OF …

Y Agca, H Men, SF Mullen, LK Riley… - Reproduction …, 2006 - CSIRO Publishing
The ability to produce porcine embryos of good quality will have a significant impact on a
number of porcine assisted reproductive technologies, such as cloning, intracytoplasmic …

Development of a culture medium (BECM-3) for porcine embryos: effects of bovine serum albumin and fetal bovine serum on embryo development

JR Dobrinsky, LA Johnson, D Rath - Biology of reproduction, 1996 - academic.oup.com
Media are available that can deliver modest porcine embryonic development from a single-
cell zygote to the blastocyst stage. However, few embryos develop to hatched blastocysts by …

Effects of fetal calf serum, amino acids, vitamins and insulin on blastocoel formation and hatching of in vivo and IVM/IVF-derived porcine embryos developing in vitro

DB Koo, NH Kim, HT Lee, KS Chung - Theriogenology, 1997 - Elsevier
The objective of this study was to determine the effects of fetal calf serum (FCS), non-
essential MEM amino acids, MEM vitamins and insulin on blastocoel formation, expansion …

Assessment of a novel media system for in vitro porcine embryo production

KA Stroble, JR Herrick, ML Conover… - Biology of …, 2002 - ansc.purdue.edu
In vitro production of porcine embryos is historically difficult. Culture systems based on the
metabolic needs of the oocyte and embryo should result in increased viability. The objective …

In vitro maturation on an agarose matrix improves the developmental competence of porcine oocytes

JE Park, M Kim, J Lee, C Chai, ST Lee, E Lee - Theriogenology, 2020 - Elsevier
Oocytes in vivo generally mature in ovarian follicles that are soft, whereas oocytes that
mature in vitro are on the hard surface of culture dishes. Embryonic ontogeny through …

Defined system for in vitro production of porcine embryos using a single basic medium

K Yoshioka, C Suzuki, A Onishi - Journal of Reproduction and …, 2008 - jstage.jst.go.jp
We have previously indicated that porcine blastocysts can be produced by in vitro
fertilization (IVF) and culture (IVC) in chemically defined porcine gamete medium (PGM) and …