[HTML][HTML] Four-tiered π interaction at the dimeric interface of HIV-1 integrase critical for DNA integration and viral infectivity
HIV-1 integrase (IN) is an essential enzyme for viral infection. Here, we report an extensive π
electron orbital interaction between four amino acids, W132, M178, F181 and F185, located …
electron orbital interaction between four amino acids, W132, M178, F181 and F185, located …
Subunit-specific protein footprinting reveals significant structural rearrangements and a role for N-terminal Lys-14 of HIV-1 Integrase during viral DNA binding
Z Zhao, CJ McKee, JJ Kessl, WL Santos… - Journal of biological …, 2008 - ASBMB
To identify functional contacts between HIV-1 integrase (IN) and its viral DNA substrate, we
devised a new experimental strategy combining the following two methodologies. First …
devised a new experimental strategy combining the following two methodologies. First …
Crystal structure of the HIV-1 integrase catalytic core and C-terminal domains: a model for viral DNA binding
JCH Chen, J Krucinski, LJW Miercke… - Proceedings of the …, 2000 - National Acad Sciences
Insolubility of full-length HIV-1 integrase (IN) limited previous structure analyses to individual
domains. By introducing five point mutations, we engineered a more soluble IN that allowed …
domains. By introducing five point mutations, we engineered a more soluble IN that allowed …
[HTML][HTML] The role of lysine 186 in HIV-1 integrase multimerization
L Berthoux, S Sebastian, MA Muesing, J Luban - Virology, 2007 - Elsevier
HIV-1 integrase (IN) catalyzes biochemical reactions required for viral cDNA insertion into
host cell chromosomal DNA, an essential step in the HIV-1 replication cycle. In one of these …
host cell chromosomal DNA, an essential step in the HIV-1 replication cycle. In one of these …
Genetic analyses of DNA-binding mutants in the catalytic core domain of human immunodeficiency virus type 1 integrase
R Lu, A Limón, HZ Ghory, A Engelman - Journal of virology, 2005 - Am Soc Microbiol
The catalytic core domain (CCD) of human immunodeficiency virus type 1 (HIV-1) integrase
(IN) harbors the enzyme active site and binds viral and chromosomal DNA during …
(IN) harbors the enzyme active site and binds viral and chromosomal DNA during …
Identifying and characterizing a functional HIV-1 reverse transcriptase-binding site on integrase
TA Wilkinson, K Januszyk, ML Phillips… - Journal of Biological …, 2009 - ASBMB
Integrase (IN) from human immunodeficiency virus, type 1 (HIV-1) exerts pleiotropic effects
in the viral replication cycle. Besides integration, IN mutations can impact nuclear import …
in the viral replication cycle. Besides integration, IN mutations can impact nuclear import …
Solution conformation and dynamics of the HIV-1 integrase core domain
NC Fitzkee, JE Masse, Y Shen, DR Davies… - Journal of biological …, 2010 - ASBMB
The human immunodeficiency virus type 1 (HIV-1) integrase (IN) is a critical enzyme
involved in infection. It catalyzes two reactions to integrate the viral cDNA into the host …
involved in infection. It catalyzes two reactions to integrate the viral cDNA into the host …
Division of labor within human immunodeficiency virus integrase complexes: determinants of catalysis and target DNA capture
TL Diamond, FD Bushman - Journal of virology, 2005 - Am Soc Microbiol
Following the completion of reverse transcription, the human immunodeficiency virus
integrase (IN) enzyme covalently links the viral cDNA to a host cell chromosome. An IN …
integrase (IN) enzyme covalently links the viral cDNA to a host cell chromosome. An IN …
Critical contribution of Tyr15 in the HIV-1 integrase (IN) in facilitating IN assembly and nonenzymatic function through the IN precursor form with reverse transcriptase
T Takahata, E Takeda, M Tobiume, K Tokunaga… - Journal of …, 2017 - Am Soc Microbiol
Nonenzymatic roles for HIV-1 integrase (IN) at steps prior to the enzymatic integration step
have been reported. To obtain structural and functional insights into the nonenzymatic roles …
have been reported. To obtain structural and functional insights into the nonenzymatic roles …
The Mobility of an HIV-1 Integrase Active Site Loop Is Correlated with Catalytic Activity,
J Greenwald, V Le, SL Butler, FD Bushman… - Biochemistry, 1999 - ACS Publications
Replication of HIV-1 requires the covalent integration of the viral cDNA into the host
chromosomal DNA directed by the virus-encoded integrase protein. Here we explore the …
chromosomal DNA directed by the virus-encoded integrase protein. Here we explore the …