Split-protein systems are invaluable tools used for the discovery and investigations of the
complexities of protein functions and interactions. Split-protein systems rely on the non …

Protein splicing in trans by split inteins has increasingly become a powerful protein-
engineering tool for protein ligation, both in vivo and in vitro. Over 100 naturally occurring …

In recent years, split inteins have seen widespread use as molecular platforms for the design
of a variety of peptide and protein chemistry technologies, most notably protein ligation. The …

Split inteins associate to trigger protein splicing in trans, a post-translational modification in
which protein sequences fused to the intein pair are ligated together in a traceless manner …

Protein splicing is an autocatalytic process involving self-excision of an internal protein
domain, the intein, and concomitant ligation of the two flanking sequences, the exteins, with …

The split inteins from the DnaE cyanobacterial family are efficient and versatile tools for
protein engineering and chemical biology applications. Their ultrafast splicing kinetics allow …

Split inteins are a class of naturally occurring proteins that carry out protein splicing in trans.
The chemical mechanism of protein trans-splicing is well-understood and has been …

Protein trans-splicing (PTS) exerted by split inteins is a protein ligation reaction which
enables overcoming the barriers of conventional heterologous protein production. We …

Split inteins carry out a naturally occurring process known as protein trans‐splicing, where
two protein fragments bind to form a catalytically competent enzyme, then catalyze their own …

Over the past decade split inteins have established themselves as powerful tools for protein
engineering, protein semisynthesis, and protein functional control approaches. Their key …