Naturally split inteins mediate a traceless protein ligation process known as protein trans‐
splicing (PTS). Although frequently used in protein engineering applications, the efficiency of …

Our knowledge of biological systems relies increasingly on the ability of quantifying and
imaging intracellular signals and events in living subjects. The development of novel …

The site-selective introduction of synthetic moieties into proteins is a highly important and
long-standing goal in many areas of biotechnology, biomedicine, and basic research. The …

Inteins are protein segments capable of joining adjacent residues via a peptide bond. In this
process known as protein splicing, the intein itself is not present in the final sequence, thus …

Obtaining insights into the molecular structure and dynamics of a protein by NMR
spectroscopy and other in-solution biophysical methods relies heavily on the incorporation …

Inteins are protein segments that are capable of enabling the ligation of flanking extein into a
new protein, a process known as protein splicing. Since its discovery, inteins have become …

Inteins are protein segments embedded in frame within a precursor sequence that catalyze
a self-excision reaction and ligate the flanking sequences with a standard peptide bond …

This volume focuses on applications of split inteins, and the progress that has been made in
the past 5 years on discovery and engineering of fast and more efficient split inteins. The first …

Inteins excise themselves out of precursor proteins by the protein splicing reaction and have
emerged as valuable protein engineering tools in numerous and diverse biotechnological …