The polymerase chain reaction (PCR) is a rapid and sensitive procedure for the in vitro
amplification of specific segments of DNA. The ability to perform primer-directed …

Previous methods for rapid isolation of total DNA for the comparative study of many fungi
have focused on obtaining a high yield of DNA for restriction enzyme analysis (Lee et al …

The polymerase chain reaction (PCR) is a powerful method with widespread applications in
molecular biology. This enzymatic reaction allows in vitro amplification of specific DNA …

The introduction of molecular biological techniques has been a major force in the areas of
systematics and population biology of filamentous fungi. Unlike the development of …

Historically, the phylogeny of fungi has been inferred from various phenotypic characters, eg
morphological, physiological and developmental characters, and/or chemical components …

Molecular approaches have become an important tool for mycologists studying fungal
systematics, molecular evolution, population genetics or plant-fungus interactions. For this …

Polymerase chain reaction (PCR) is a technique widely used in fungal research. One of its
advantages is the ability to amplify very small amounts of DNA, in the picograms range, even …

DNA isolation from some fungal organisms of agronomic importance is difficult because they
have cell walls or capsules that are relatively unsusceptible to lysis. We have developed a …

Analysis of restriction fragment length polymorphisms (RFLPs) has become an important
approach to studying population genetics, molecular evolution, and systematics offungi …

Gene replacement via homologous double crossover in filamentous fungi requires relatively
long (preferentially> 0.5 kb) flanking regions of the target gene. For this reason, gene …