Gene expression occurs in two essential steps: transcription and translation. In bacteria, the
two processes are tightly coupled in time and space, and highly regulated. Tight regulation …

Insights from novel mechanistic paradigms in gene expression control have led to the
development of new gene expression systems for bioproduction, control, and sensing …

Gene expression is regulated by specific transcriptional circuits but also by the global
expression machinery as a function of growth. Simultaneous specific and global regulation …

Despite enormous progress in understanding the fundamentals of bacterial gene regulation,
our knowledge remains limited when compared with the number of bacterial genomes and …

In synthetic biology, researchers assemble biological components in new ways to produce
systems with practical applications. One of these practical applications is control of the flow …

Robust and predictably performing synthetic circuits rely on the use of well-characterized
regulatory parts across different genetic backgrounds and environmental contexts. Here we …

Highlights•Predictability and robustness are major challenges for synthetic
biology.•Engineering bacteria requires putting synthetic constructs into growing host …

Genetic circuits and metabolic pathways can be reengineered to allow organisms to process
signals and manufacture useful chemicals. However, their functions currently rely on …

Here, we determined the relative importance of different transcriptional mechanisms in the
genome-reduced bacterium Mycoplasma pneumoniae, by employing an array of …

Organisms orchestrate cellular functions through transcription factor (TF) interactions with
their target genes, although these regulatory relationships are largely unknown in most …