A good part of the contemporary synthetic biology agenda aims at reprogramming
microorganisms to enhance existing functions and/or perform new tasks. Moreover, the …

Highlights•Genes and genetic circuits exhibit growth-rate dependence.•Growth rate
dependence reflects coupling of gene expression and physiological state of the cell.•Several …

Bacteria evolved many strategies to survive and persist within host cells. Secretion of
bacterial effectors enables bacteria not only to enter the host cell but also to manipulate host …

Cell‐free expression systems enable rapid prototyping of genetic programs in vitro.
However, current throughput of cell‐free measurements is limited by the use of channel …

As bacteria and fungi have been found to contain genes encoding enzymes that synthesize
a plethora of potential secondary metabolites, interest has grown in the activation of these …

Summary The XylS/Pm regulator/promoter system originating from the Pseudomonas putida
TOL plasmid pWW 0 is widely used for regulated low‐and high‐level recombinant …

Most bacterial promoters are regulated by several signals. This is reflected in the complexity
of their organization, with multiple binding sites for different transcription factors. Studies of a …

Networks of molecular regulators are often the primary objects of focus in the study of gene
regulation, with the machinery of protein synthesis tacitly relegated to the background …

Highlights•Microbial production of valuable chemicals can be improved by dynamic
regulation.•Dynamic regulation balances flux between growth of cell and production of …

Signal transduction pathways control most cellular activities in living cells ranging from
regulation of gene expression to fine-tuning enzymatic activity and controlling motile …