Precise, analogue regulation of gene expression is critical for cellular function in mammals.
In contrast, widely employed experimental and therapeutic approaches such as knock-in/out …

New technologies are emerging that utilize artificial microRNA (miRNA) target sites to exploit
or inhibit endogenous miRNA regulation. This approach has been used to improve cell …

The ability to express transgenes at specified levels is critical for understanding cellular
behaviors, and for applications in gene and cell therapy. Transfection, viral vectors, and …

As molecular and cellular therapies advance in the clinic, the role of genetic regulation is
becoming increasingly important for controlling therapeutic potency and safety. The …

We have shown previously that transgene expression can be suppressed in hematopoietic
cells using vectors that are responsive to microRNA (miRNA) regulation. Here we …

Abstract Owing to Karl Landsteiner's discovery of blood groups, blood transfusions became
safe cellular therapies in the early 1900s. Since then, cellular therapy made great advances …

The discovery of RNAi has revolutionized loss-of-function genetic studies in mammalian
systems. However, significant challenges still remain to fully exploit RNAi for mammalian …

RNAi is proving to be a powerful experimental tool for the functional annotation of
mammalian genomes. The full potential of this technology will be realized through …

Synthetic mRNA circuits manipulate cell fate by controlling output protein expression via cell-
specific input molecule detection. Most current circuits either repress or enhance output …

Competing endogenous RNAs (ceRNAs) cross-regulate each other at the
posttranscriptional level by titrating shared microRNAs (miRNAs). Here, we established a …