Antibody specific to the alpha subunit of the guanine nucleotide-binding regulatory protein Go: developmental appearance and immunocytochemical localization in …

KJ Chang, W Pugh, SG Blanchard… - Proceedings of the …, 1988 - National Acad Sciences
A polyclonal rabbit antibody (9120) against the alpha subunit of the" other" guanine
nucleotide-binding protein Go (alpha o) was raised against a synthetic alpha o peptide …

Use of specific antibodies to quantitate the guanine nucleotide-binding protein Go in brain.

P Gierschik, G Milligan, M Pines… - Proceedings of the …, 1986 - National Acad Sciences
We immunized rabbits with purified guanine nucleotide-binding proteins (G proteins) from
bovine brain and obtained an antiserum, RV3, that reacts specifically with the alpha subunit …

Specific antibodies against Go isoforms reveal the early expression of the Go2 alpha subunit and appearance of Go1 alpha during neuronal differentiation.

B Rouot, N Charpentier, C Chabbert, J Carrette… - Molecular …, 1992 - ASPET
We have previously identified two isoforms of Go alpha in membranes of N1E-115
neuroblastoma cells, using an antibody raised against the purified Go alpha subunit; one …

Immunocytochemical localization of the guanine nucleotide-binding protein Go in primary cultures of neuronal and glial cells

P Brabet, A Dumuis, M Sebben… - Journal of …, 1988 - Soc Neuroscience
We have localized the guanine nucleotide-binding protein, Go, in primary cultures of pure
neuronal and glial cells prepared from different mouse brain areas. Immunoblotting …

Ontogeny of the GTP‐Binding Protein Go in Rat Brain and Heart

T Asano, N Kamiya, R Semba… - Journal of …, 1988 - Wiley Online Library
We determined the ontogeny of the GTP‐binding protein Go in rat brain and heart by
employing highly sensitive enzyme immunoassay methods. In the brain, the α subunit of Go …

Neuroblastoma Differentiation Involves the Expression of Two Isoforms of the α‐Subunit of Go

P Brabet, C Pantaloni, M Rodriguez… - Journal of …, 1990 - Wiley Online Library
The regulation of GTP‐binding proteins (G proteins) was examined during the course of
differentiation of neuroblastoma N1E‐115 cells. N1E‐115 cell membranes possess three …

Go, a guanine nucleotide-binding protein: immunohistochemical localization in rat brain resembles distribution of second messenger systems.

PF Worley, JM Baraban, C Van Dop… - Proceedings of the …, 1986 - National Acad Sciences
We have localized a guanine nucleotide-binding protein, Go, in rat brain by
immunohistochemistry with a selective polyclonal antiserum to the alpha 39 subunit of Go …

Structure of the human gene and two rat cDNAs encoding the alpha chain of GTP-binding regulatory protein Go: two different mRNAs are generated by alternative …

T Tsukamoto, R Toyama, H Itoh… - Proceedings of the …, 1991 - National Acad Sciences
Go is a specific class (" other") of signal-transducing heterotrimeric GTP-binding proteins (G
proteins) that is expressed in high levels in mammalian brain. We have cloned two different …

Metabolism of two Go alpha isoforms in neuronal cells during differentiation

P Brabet, C Pantaloni, J Bockaert… - Journal of Biological …, 1991 - Elsevier
We have previously shown that undifferentiated N1E-115 neuroblastoma cells express only
one isoform of Go alpha (pI= 5.8), whereas differentiated neuroblastoma cells expressed, in …

Alternative splicing produces transcripts encoding two forms of the alpha subunit of GTP-binding protein Go.

M Strathmann, TM Wilkie… - Proceedings of the …, 1990 - National Acad Sciences
The alpha subunit of the guanine nucleotide-binding protein Go (" o" for other) is believed to
mediate signal transduction between a variety of receptors and effectors. cDNA clones …