Cas9-NG greatly expands the targeting scope of the genome-editing toolkit by recognizing NG and other atypical PAMs in rice
B Ren, L Liu, S Li, Y Kuang, J Wang, D Zhang, X Zhou… - Molecular plant, 2019 - cell.com
CRISPR technologies enabling precise genome manipulation are valuable for gene function
studies and molecular crop breeding. However, the requirement of a protospacer adjacent …
studies and molecular crop breeding. However, the requirement of a protospacer adjacent …
Expanding the range of CRISPR/Cas9 genome editing in rice
The CRISPR/Cas9 system has emerged as a versatile molecular tool for genome editing in
various organisms, including plants. In this system, the specificity of Cas9-directed DNA …
various organisms, including plants. In this system, the specificity of Cas9-directed DNA …
[PDF][PDF] Robust genome editing of CRISPR-Cas9 at NAG PAMs in rice
The CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats/Cas9) system
has been widely used for a variety of applications, including targeted gene knockout, gene …
has been widely used for a variety of applications, including targeted gene knockout, gene …
[HTML][HTML] Targeted base editing in rice with CRISPR/ScCas9 system
M Wang, Z Xu, G Gosavi, B Ren, Y Cao… - Plant Biotechnology …, 2020 - ncbi.nlm.nih.gov
The CRISPR/Cas system has rapidly become the preferred tool for genome engineering in
various organisms due to high efficiency, specificity, simplicity and versatility. Currently …
various organisms due to high efficiency, specificity, simplicity and versatility. Currently …
Genome engineering in rice using Cas9 variants that recognize NG PAM sequences
K Hua, X Tao, P Han, R Wang, JK Zhu - Molecular plant, 2019 - cell.com
CRISPR/Cas9 genome editing relies on sgRNA-target DNA base pairing and a short
downstream PAM sequence to recognize target DNA. The strict protospacer adjacent motif …
downstream PAM sequence to recognize target DNA. The strict protospacer adjacent motif …
Genome editing in plants by engineered CRISPR–Cas9 recognizing NG PAM
Abstract Streptococcus pyogenes Cas9 (SpCas9) is widely used for genome editing and
requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered …
requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered …
Expanding the scope of CRISPR/Cpf1-mediated genome editing in rice
The CRISPR/Cas gene editing system offers great potential for functional genomics in plants
and crop improvement. The specificity of Cas-directed DNA cleavage is strictly determined …
and crop improvement. The specificity of Cas-directed DNA cleavage is strictly determined …
SpRY greatly expands the genome editing scope in rice with highly flexible PAM recognition
Z Xu, Y Kuang, B Ren, D Yan, F Yan, C Spetz, W Sun… - Genome biology, 2021 - Springer
Background Plant genome engineering mediated by various CRISPR-based tools requires
specific protospacer adjacent motifs (PAMs), such as the well-performed NGG, NG, and …
specific protospacer adjacent motifs (PAMs), such as the well-performed NGG, NG, and …
Recent advances in genome editing using CRISPR/Cas9
Y Ding, H Li, LL Chen, K Xie - Frontiers in plant science, 2016 - frontiersin.org
The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-
associated nuclease 9) system is a versatile tool for genome engineering that uses a guide …
associated nuclease 9) system is a versatile tool for genome engineering that uses a guide …
Expanding the scope of CRISPR/Cas9‐mediated genome editing in plants using an xCas9 and Cas9‐NG hybrid
Q Niu, S Wu, Y Li, X Yang, P Liu, Y Xu… - Journal of integrative …, 2020 - Wiley Online Library
The widely used Streptococcus pyogenes Cas9 (SpCas9) requires NGG as a protospacer
adjacent motif (PAM) for genome editing. Although SpCas9 is a powerful genome‐editing …
adjacent motif (PAM) for genome editing. Although SpCas9 is a powerful genome‐editing …