Tritiated thymidine labelling for various times together with Feulgen microspectrophotometry
was used to determine the presence of different cell lines in monolayer cell cultures. Using …

Anumber of methods are available for the determination of the durations of pre-DNA
synthesis period (G,), DNA synthesis period (S) and post-DNA synthesis period (Ge) of …

IN ORDER TO determine the number of proliferating cells, the labelling index is frequently
measured by autoradiography after incubation of the cells with 3H-thymi-dine. For an …

We report the development and characterization of a semiautomated method for
measurement of cell proliferation in microculture using Hoechst 33342, a non-toxic specific …

Thymidine kinase is a key enzyme for the application of drugs in chemotherapy and for
diagnosis. Although of great interest, its regulation during cell cycle and differentiation is …

Untransformed Syrian hamster fibroblasts in short-term culture were studied by means of
[3H] thymidine autoradiography combined with differential staining after continuous …

A method is described to combine tritiated thymidine autoradiography with
immunoperoxidase detection of bromodeoxyuridine on the same paraffin sections. It …

A method is described for the rapid, accurate measurement of single cells in situ using
microfluorimetry. This method involves a shutter system, which allows irradiation of single …

A simple, reproducible fluorescent assay has been described which can be used confidently
at microgram and submicrogram levels. The method is most suitable for cell cycle and cell …

A method of improving absorption cytophotometric cellular DNA values by making
measurements on Feulgenstained cells at optimal stain absorbances has been developed …