Free radical scavengers ameliorate the 2-cell block in mouse embryo culture

M Legge, MH Sellens - Human Reproduction, 1991 - academic.oup.com
Mouse zygotes from a strain combination which fails to undergo cleavage in vitro (the 2-cell
block) were cultured in a variety of media, using either tow (5%) or normal (20%) oxygen …

Evaluation of mouse preimplantation embryos exposed to oxidative stress cultured with insulin-like growth factor I and II, epidermal growth factor, insulin, transferrin …

R Kurzawa, W Głabowski, T Baczkowski… - Reproductive …, 2002 - europepmc.org
Blastocyst culture requires strictly defined culture media to sustain its viability and quality.
Although blastocyst media are commercially available, they do not meet all the needs and …

Quantitative analysis of cellular glutathione in early preimplantation mouse embryos developing in vivo and in vitro

MH Nasr-Esfahani, MH Johnson - Human Reproduction, 1992 - academic.oup.com
The relative levels of reduced glutathione (GSH) have been measured fluorimetrically in
individual eggs and early embryos from two mouse strains, one of which shows …

Glutathione redox cycle-driven recovery of reduced glutathione after oxidation by tertiary-butyl hydroperoxide in preimplantation mouse embryos

CS Gardiner, DJ Reed - Archives of biochemistry and biophysics, 1995 - Elsevier
The purpose of this study was to determine whether mouse embryos at the two-cell to
blastocyst stage have the capacity to reduce glutathione disulfide (GSSG) and to elucidate …

Combined effects of individual culture and atmospheric oxygen on preimplantation mouse embryos in vitro

RL Kelley, DK Gardner - Reproductive Biomedicine Online, 2016 - Elsevier
Embryos are routinely cultured individually, although this can reduce blastocyst
development. Culture in atmospheric (20%) oxygen is also common, despite multiple …

Effects of glucose, glutamine, ethylenediaminetetraacetic acid and oxygen tension on the concentration of reactive oxygen species and on development of the mouse …

MH Nasr-Esfahani, NJ Winston, MH Johnson - Reproduction, 1992 - rep.bioscientifica.com
Analysis over the first 48 h of development in vitro from the one-cell stage to the early four-
cell stage indicated that (i) ethylenediaminetetraacetic acid (EDTA) exerts the major …

Antiapoptotic and embryotrophic effects of α-tocopherol and L-ascorbic acid on porcine embryos derived from in vitro fertilization and somatic cell nuclear transfer

YW Jeong, SW Park, MS Hossein, S Kim, JH Kim… - Theriogenology, 2006 - Elsevier
The objective was to determine optimal concentrations of α-tocopherol and l-ascorbic acid
for development of porcine embryos derived from in vitro-fertilization (IVF) or somatic cell …

Protein supplementation and the incidence of apoptosis and oxidative stress in mouse embryos

N Esfandiari, T Falcone, A Agarwal… - Obstetrics & …, 2005 - journals.lww.com
OBJECTIVE: To estimate the effect of protein supplementation of culture media on reactive
oxygen species production and incidence of apoptosis in preimplantation mouse embryos …

[HTML][HTML] Reactive oxygen species generation and use of antioxidants during in vitro maturation of oocytes

M Khazaei, F Aghaz - International journal of fertility & sterility, 2017 - ncbi.nlm.nih.gov
In vitro maturation (IVM) is emerging as a popular technology at the forefront of fertility
treatment and preservation. However, standard in vitro culture (IVC) conditions usually …

Antioxidants supplementation improves the quality of in vitro produced ovine embryos with amendments in key development gene expressions

SK Tripathi, S Nandi, PSP Gupta, S Mondal - Theriogenology, 2023 - Elsevier
The present study assessed the effects of supplementation of different antioxidants on
oocyte maturation, embryo production, reactive oxygen species (ROS) production and …