Over the past decade split inteins have established themselves as powerful tools for protein
engineering, protein semisynthesis, and protein functional control approaches. Their key …

Protein trans-splicing (PTS) exerted by split inteins is a protein ligation reaction which
enables overcoming the barriers of conventional heterologous protein production. We …

Protein trans-splicing by split inteins has many uses in protein production and research.
Splicing proteins with synthetic peptides, which employs atypical split inteins, is particularly …

Split inteins are a class of naturally occurring proteins that carry out protein splicing in trans.
The chemical mechanism of protein trans-splicing is well-understood and has been …

The protein trans-splicing (PTS) activity of naturally split inteins has found widespread use in
chemical biology and biotechnology. However, currently used naturally split inteins suffer …

Split inteins carry out a naturally occurring process known as protein trans‐splicing, where
two protein fragments bind to form a catalytically competent enzyme, then catalyze their own …

Inteins excise themselves out of precursor proteins by the protein splicing reaction and have
emerged as valuable protein engineering tools in numerous and diverse biotechnological …

Protein splicing in trans by split inteins has increasingly become a powerful protein-
engineering tool for protein ligation, both in vivo and in vitro. Over 100 naturally occurring …

Inteins catalyze a protein splicing reaction to excise the intein from a precursor protein and
join the flanking sequences (exteins) with a peptide bond. In a split intein, the intein …

Protein trans-splicing by split inteins holds great potential for the chemical modification and
semisynthesis of proteins. However, the structural requirements of the extein sequences …